摘要
花色性状是甘蓝的一个重要性状,在吸引和指示传粉者、保护花器官、维持花组织能量平衡、测定品种异交率及纯度、检测性状转移等方面有重要作用。为了鉴定控制和影响甘蓝花色的遗传位点和候选基因,本研究利用芥蓝(白花)与野生甘蓝(黄花)构建了F2分离群体,并分别利用基于分子标记遗传连锁图谱和基于SNP芯片分析的QTL扫描技术,对甘蓝花色性状进行QTL定位。本研究结论如下:甘蓝花色性状由C03染色体上一个部分显性主效QTL位点控制,并受到C02上一个微效加性QTL的影响;主效QTL候选基因BoCCD4编码区778~780 bp处3个碱基的插入极可能导致BoCCD4功能丧失,从而呈现黄色表型。本研究确认了控制甘蓝白花性状的主效QTL位置,鉴定到了候选基因并发现了与文献报道不同的新变异位点,为进一步了解芸薹属物种花色的遗传和变异提供了新的数据。
Flower color is an important trait in Brassica oleracea L.which can attract and guide the insect pollinators,protect flower organs,maintain the energy homeostasis in flower,reveal the rate and purity of out-crossing and indicate the introgressions of traits.In order to identify the genetic loci and candidate genes controlling flower color in B.oleracea L.,we constructed an F2 population from the cross between a B.alboglabra Bailey line(white flower)and a wild B.oleracea L.accession(yellow flower),and conducted QTL analysis using a previous molecular-marker-based genetic linkage map and a SNP microarray.Our results suggest that the white flower in B.oleracea L.is controlled by a partial dominant locus on chromosome C03 and possibly regulated by a minor additive QTL on C02.Furthermore,the 3-bp insertion in the candidate gene BoCCD4 was assumed to result in the loss of function of BoCCD4,leading to a yellow flower.Thus,this study identified a novel allele of the candidate gene controlling the flower color in B.oleracea L.,and this result might contribute to understand the variation and evolution of flower color in Brassica species.
作者
杨茹涵
刘进
李加纳
钱伟
梅家琴
YANG Ru-han;LIU Jin;LI Jia-na;QIAN Wei;MEI Jia-qin(Southwest University College of Agronomy and Biotechnology,Chongqing 400715;Southwest University Academy of Agricultural Sciences,Chongqing 400715)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第5期1271-1277,共7页
Journal of Plant Genetic Resources
基金
国家重点研发计划项目(2018YFD0100501)
国家油菜产业体系(CARS-12)
重庆市社会事业与民生保障科技创新项目(cstc2017shms-xdny80050)~~
关键词
甘蓝
花色
数量性状位点
BoCCD4
变异分析
Brassica oleracea L.
flower color
quantitative trait loci
BoCCD4
variation analysis
作者简介
第一作者:杨茹涵,研究方向为植物分子育种,E-mail:2273683100@qq.com;通信作者:梅家琴,研究方向为分子育种,E-mail:jiaqinmay@163.com
