摘要
为阐明肌间刺缺失对斑马鱼(Danio rerio)胚胎发育时期肌肉发育的影响,采用qRT-PCR的方法,检测分析了斑马鱼不同肌间刺表型间5个肌肉特异性基因(mef2、myf5、myod、myog和sox6)在囊胚期(3hpf,hourspost fertilization)、原肠胚期(6 hpf)、体节期(12 hpf)、咽囊期(24 hpf)和孵化期(72 hpf)5个发育时期的表达情况,对比分析了肌间刺缺失对斑马鱼胚胎发育过程中肌肉发育特异性基因的时序表达差异和肌肉发育的影响,以及对受精率、孵化率和畸形率的影响。结果显示, 5个特异性基因在肌间刺缺失突变型与野生型斑马鱼各发育时期的表达量无显著性差异(P>0.05)。其中,myf5、myod基因在原肠胚期表达量升高,在体节期和咽囊期开始逐步下降;mef2、myog和sox6基因在囊胚期和原肠胚期表达量很低,在体节期和咽囊期表达量迅速升高,并在孵化期开始下降。此外,受精率、孵化率和畸形率的统计结果显示,肌间刺突变型与野生型间亦无显著性差异(P>0.05)。综上,推测肌间刺的缺失对斑马鱼胚胎发育及胚胎发育过程中肌肉的发育没有影响。
In order to explore the difference in embryonic muscle development between an intermuscular bone(Imb)deficiency mutant and wildtype zebrafish,we used a qRT-PCR method to analyze the expression of 5 muscle-specific genes,including mef2,myf5,myod,myog,and sox6,in the development stages blastula(3 hpf,hours post fertilization),gastrula(6 hpf),segmentation(12 hpf),pharyngula(24 hpf),and hatching period(72 hpf),and discussed the impact of Imb’s deficiency on muscle development during embryonic development.Furthermore,the differences in fertilization rate,hatching,and abnormal rate of larvae were assessed.The results showed that the expression of genes myf5 and myod were elevated in the gastrula stage,and downregulated in the segmentation and pharyngula stages;the expression levels of mef2,myog,and sox6 in the blastula and gastrula stages were extremely low,and were upregulated significantly in the segmentation and pharyngula stages before being downregulated in the hatching period.The results also showed that there were no significant differences in 5 muscle-specific genes’expression between the wildtype zebrafish and its Imb deficiency mutant.Additionally,no significant difference in fertilization rate,hatching,and abnormal rate occurred between the two Imb phenotypes.In summary,the deletion of Imb do not impact muscle development or the survival of embryos during embryonic development of zebrafish.
作者
杨建
佟广香
郑先虎
孙志鹏
吕伟华
孙效文
匡友谊
YANG Jian;TONG Guangxiang;ZHENG Xianhu;SUN Zhipeng;LYU Weihua;SUN Xiaowen;KUANG Youyi(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2019年第2期296-303,共8页
Journal of Fishery Sciences of China
基金
中国水产科学研究院基本科研业务费项目(2016HY-ZD0301)
关键词
肌间刺
斑马鱼
胚胎发育
肌肉
Imb
zebrafish
embryonic development
muscle
作者简介
杨建(1987-),男,博士研究生,主要从事鱼类遗传育种的研究.E-mail:last0templar@126.com;通信作者:匡友谊,副研究员,主要从事鱼类基因组与分子育种研究.E-mail:kuangyouyi@hrfri.ac.cn
