摘要
以收集于黄土高原的8种小粒黑大豆为实验材料,4种小粒黄大豆为对照,采用碱溶酸沉法提取蛋白质,对其蛋白质的功能特性进行分析。结果表明,8种小粒黑大豆蛋白的氮溶解指数、吸水性、吸油性、乳化能力及乳化稳定性、起泡能力、凝胶强度在51. 72%~62. 07%、2. 29~2. 95 g/g、1. 52~1. 70 g/g、54. 40%~88. 60%、83. 35%~97. 16%、75. 70%~95. 80%、154. 59~203. 66 g之间。小粒黑大豆蛋白的氮溶解指数和吸油性显著低于小粒黄大豆;盐池黑豆、定边小黑豆和靖边王渠子黑豆的蛋白质吸水性显著高于小粒黄大豆;乳化能力及乳化稳定性均优于小粒黄大豆,起泡性和泡沫稳定性差于粒小粒黄大豆;除子洲小黑豆、横山老黑豆和偏关小黑豆外,小粒黑大豆蛋白的凝胶强度显著高于小粒黄大豆。不同品种间小粒黑大豆蛋白质的氮溶解指数、吸水性、吸油性、乳化稳定性、起泡能力及凝胶强度有显著差异。
The eight kinds of small black soybeans collected from the Loess Plateau were used as test materials,and four kinds of small yellow soybeans were used as controls. Protein was extracted by alkali-solution and acid-isolation, and the functional properties of the protein were analyzed. The results showed that nitrogen soluble index,water-absorbing ability, oil-absorbing ability, emulsification and emulsion stability, foaming ability and gel strength of 8 kinds of small soybeans protein varied from 51.72% to 62. 07%,2. 29 to 2. 95 g/g,1. 52 to 1.70 g/g,54.40% to 88. 60%,83. 35% to 97.16%,75. 70% to 95. 80% and 154.59 to 203.66 g, respectively. Compared with 4 kinds of small yellow soybeans protein, the nitrogen soluble index and oil-absorbing ability of small black soybeans protein were significantly lower than small yellow soybeans. The water-absorbing ability of Yanchi, Dingbian and Wangquzi small black soybeans protein was significantly higher than small yellow soybeans, the emulsification and emulsion stability were higher than small yellow soybeans, the foaming ability and foam stability were lower than small yellow soybeans, the gel strength was higher than small yellow soybeans except for Zizhou, Hengshan and Pianguan small black soybeans. There were significant differences in nitrogen soluble index, water-absorbing ability, oil-absorbing ability, emulsification stability, foaming ability and gel strength of different small black soybeans protein.
作者
江帆
郭颖
王华
梁鸡保
杜双奎
Jiang Fan;Guo Ying;Wang Hua;Liang Jibao;Du Shuangkui(College of Food Science and Engineering,Northwest A&F University,Yangling 712100;Detachment of food law-enforcement monitoring in Weinan,Weinan 714000;Agro-Technical Promotion Center,Shenmu 719300)
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2019年第2期61-66,共6页
Journal of the Chinese Cereals and Oils Association
基金
陕西省重点研发计划(2017NY-177)
榆林市科技计划(2016-19-2)
关键词
小粒黑大豆
小粒黄大豆
分离蛋白
功能特性
small black soybean
small yellow soybean
protein isolate
functional properties
作者简介
江帆,女,1994年出生,硕士,黑豆加工性能评价;通信作者:杜双奎,男,1972年出生,教授,杂粮资源开发与利用。