摘要
目的研究人脑胶质瘤石蜡包埋组织(formalin-fixed paraffin-embedded,FFPE)中微小RNA-184(microRNA-184,miR-184)的表达及其与临床病理特征之间的关系,探讨其在预测胶质瘤患者预后中的价值。方法采用微阵列芯片法和RT-PCR检测108例胶质瘤FFPE和32例正常脑组织中的mi R-184表达,同时Kaplan-Meier法分析胶质瘤患者总生存期和无病生存期情况。结果胶质瘤组织中miR-184的表达下调,并用RT-PCR进一步验证;miR-184低表达与WHO分级(P=0.007)、Karnofsky评分(KPS)(P=0.029)、复发时间(P=0.037)和生存时间(P=0.019)显著相关;Kaplan-Meier分析结果表明,miR-184低表达与高表达患者总生存期(OS)(P=0.001)和无病生存期(DFS)(P=0.006)差异有统计学意义,mi R-184低表达患者预后较差。多因素分析显示胶质瘤中miR-184的差异表达是预测OS[风险比(HR):7.52;95%CI:2.63~21.42;P=0.002]和DFS[HR:11.56;95%CI:5.17~25.93;P<0.001]的独立危险因素。结论 miR-184的表达与胶质瘤患者预后显著相关,表明miR-184可作为预测胶质瘤患者预后的独立标志物。
Objective To investigate the relationship between microRNA-184(mi R-184) expression and clinicopathologic factors in glioma formalin-fixed paraffin-embedded(FFPE) tissues, and to evaluate the role of mi R-184 expression in predicting the prognosis of glioma patients. Methods The expression levels of mi R-184 were analyzed in 108 gliomas FFPE and 32 normal brain tissues by microarray and real-time PCR(RT-PCR). Survival analysis by Kaplan-Meier method was performed to assess prognostic significance. Results Down-regulation of mi R-184 was detected in most primary glioma tissues, which was confirmed by RT-PCR analysis further. Additionally, down-regulation of mi R-184 was significantly associated with WHO grade(P=0.007), Karnofsky performance score(KPS)(P=0.029), time to recurrence(P=0.037) and survival time(P=0.019). Using Kaplan-Meier analysis, a comparison of survival curves of low high expresser of mi R-184 revealed a highly significant difference in overall survival(OS)(P=0.001) and disease-free survival(DFS)(P=0.006), which suggested that low expression of mi R-184 was associated with a worse prognosis. Multivariate analyses showed that mi R-184 expression was the independent risk factors for predicting OS [hazard ratio(HR), 7.52; 95%CI: 2.63-21.42; P=0.002] and DFS[HR, 11.56; 95%CI: 5.17-25.93; P<0.001] of glioma patients. Conclusion miR-184 expression is significantly correlated with the prognosis of glioma patients, suggesting that miR-184 may serve as the independent prognostic marker.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2015年第5期483-487,共5页
Cancer Research on Prevention and Treatment
