摘要
目的分析2016-2017马鞍山市流感病毒病原学监测结果,为流感防制提供科学依据。方法收集、分析马鞍山市2016-2017年流感样病例(influenza-like illness,ILI)监测数据,同时采集部分ILI咽拭子标本,采用RT-PCR法检测流感病毒核酸,对病原学检测数据进行统计分析。结果 2016年共检测ILI咽拭子标本2 772份,流感病毒核酸检测阳性383例,总阳性率为13.82%。其中新甲型H1N1亚型59例(占15.40%),H3N2亚型111例(占28.98%),B-Yamagata亚型11例(占2.87%),B-Victoria亚型202例(占52.74%);2017年共检测ILI咽拭子标本2 170份,流感病毒核酸检测阳性614例,总阳性率为28.29%,其中新甲型H1N1亚型38例(占52.74%),H3N2亚型309例(占50.33%),B-Yamagata亚型178例(占28.99%),B-Victoria亚型89例(占14.50%)。不同月份阳性检出率比较差异有统计学意义(年X_(2016)~2=450.876,X_(2017)~2=240.002,P均<0.05)。核酸检测阳性率性别差异无统计学意义(X_(2016)~2=0.171,X_(2017)~2=0.174,P均>0.05)。不同年龄组间阳性率比较差异有统计学(X_(2016)~2=160.030,X_(2017)~2=132.901,P均<0.05)。2016、2017年MDCK细胞培养法分离流感病毒221株和98株,分离率分别为57.70%和15.96%;2016、2017年鸡胚培养法分离流感病毒26株和8株,分离率分别为6.79%和1.30%。两种方法比较有统计学差异(X^2=74.815,P<0.05)。结论 2016年马鞍山市流感呈现冬春季两个发病高峰,主要流行株为B-Victoria型;2017年出现夏、冬、春季3个发病高峰,主要流行株为H3N2型。流行株发病高峰的变化可为当地流感防控提供参考,流感监测应进一步加强。
Objective To analyze the results of influenza surveillance in the City of Maanshan from 2016 to 2017 in order to provide scientific evidence for influenza prevention. Methods Data on cases of influenza-like illness(ILI) in the City of Maanshan from 2016 to 2017 were collected and analyzed. Throat swabs were also collected from individuals with ILI. Nucleic acids of influenza virus were detected using RT-PCR and results were statistically analyzed. Results In 2016, a total of 2,772 throat swabs from individuals with ILI were tested, and 383 tested positive for nucleic acids of influenza virus. Swabs tested positive at a rate of 13.82%. Of the specimens that tested positive, 59(15.40%) were new subtype HIN1, 111(28.98%) were subtype H3N2, 11(2.87%) were subtype B-Yamagata, and 202(52.74%) were subtype B-Victoria. In 2017, a total of 2,170 throat swabs from individuals with ILI were tested, and 614 tested positive for nucleic acids of influenza virus. Swabs tested positive at a rate of 28.29%, including 38(52.74%) with the new influenza A virus subtype H1 N1, 309(50.33%) with the subtype H3 N2, 178(28.99%) with the subtype B-Yamagata, and 89(14.50%) with the subtype B-Victoria. The rate of detection differed significantly in different months( of 450.876 in 2016, 240.002 in 2017, P<0.05). The rate of nucleic acid detection did not differ significantly( of 0.171 in 2016, 0.174 in 2017, P>0.05). The rate of detection of nucleic acids of influenza virus differed significantly among different age groups( of 160.030 in 2016, 132.901 in 2017, P<0.05). Two hundred and twenty-one strains of influenza virus were isolated in 2016(57.70%) using the MDCK cell line, and 98 strains were similarly isolated(15.96%) in 2017. Twenty-six strains of influenza virus were isolated(6.79%) using chicken embryos in 2016, and 8 strains were similarly isolated(1.30%) in 2017. The rate of isolation using the MDCK cell line and chicken embryos differed significantly(X^2=74.815, P<0.05). Conclusion In 2016, influenza in the City of Maanshan peaked twice, once in the winter and once in the spring. Influenza was mainly caused by subtype B-Victoria. In 2017, there were three peaks in the summer, winter, and spring. Influenza was mainly caused by subtype H3 N2. Changes in the peak incidence of prevalent strains may provide a reference for local influenza prevention and control. Surveillance of influenza should be further enhanced.
作者
张云霞
江良梁
赵志荣
ZHANG Yun-xia;JIANG Liang-liang;ZHAO Zhi-rong(Center for Disease Control and Prevention of Maanshan,City of Maanshan,Anhui,China 243011)
出处
《中国病原生物学杂志》
CSCD
北大核心
2019年第2期194-198,共5页
Journal of Pathogen Biology
关键词
流感病毒
亚型
分离培养
监测
Influenza virus
subtype
isolation
surveillance
作者简介
通讯作者:张云霞(1980-),女,安徽当涂人,硕士,主管技师。主要研究方向:呼吸道传染病监测与检测。E-mail:yunxiazhang0610@163.com.
