摘要
测定了四川和广西产山豆根中苦参碱的含量.采用离子对色谱法,色谱柱为Shimpack CLC-ODS(150×6mm i.d.),流动相为CH3CN:0.025mol·L-1(NH4)2SO4:SDS=34:66:0.6(W/W),用20％H2SO4调pH=3.0.同时对样品的提纯条件、测试结果的稳定性、精密度与重现性进行了研究.结果表明四川产山豆根苦参碱含量为0.305％,广西产山豆根中苦参碱的含量0.210％.
The matrine contents in sophore tonkinesis gapnep grown in Sichuan province and Guangxi province are determined by HPLC using Shimack CLC-ODS column(150×6mm i.d.) with CH3CN:0.025mol·L-1(NH4)2SO4:SDS = 34:66:0.6(W/W) as mobile at pH=3.0. The extraction condition for the samples, stability and precision of the method are also investigated. The results indicate that the matrine content of the sample from Sichuan is 0.305% and 0.210% from Guangxi, respectively.
出处
《西南民族大学学报（自然科学版）》
CAS
2003年第2期171-173,共3页
Journal of Southwest Minzu University(Natural Science Edition)
