摘要
DNA宏条形码(metabarcoding)技术是一项传统DNA条形码与高通量测序技术相结合后衍生出的研究方法,该技术可快速、简便、有效地从多物种混合的生物样本中识别、还原其中的生物种类,目前在环境生物学研究中得到广泛运用。在市售中成药领域,掺伪、质量不稳定等因素严重制约了相关产业的可持续发展。该文介绍了DNA宏条形码技术的相关背景及其在中成药鉴定中的初步应用情况,同时还简述了研究过程中可能存在的问题并对DNA宏条形码技术发展进行了展望。
Metabarcoding technology is a research method derived from the combination of traditional DNA barcodes and highthroughput sequencing technologies. It can quickly,easily and efficiently identify and restore biological samples from multiple species.Biological species are currently widely used in environmental biology research. In the market of traditional Chinese medicines,adulteration and quality instability have severely restricted the sustainable development of the related industries. This article introduced the background of the metabarcoding technology and its preliminary application in the identification of Chinese patent medicines. It also outlined the possible problems in the research process and prospected to the development of the DNA metabarcoding technology.
作者
高玉珍
韦瑾
刘振稳
周静
GAO Yu-zhen;WEI Jin;LIU Zhen-wen;ZHOU Jing(School of Pharmaceutical Science&Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming 650500,China;Key Laboratory for Plant Diversity and Biogeography of East Asia,Kunming Institute of Botany,Chinese Academy of Science,Kunming 650201,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第2期261-264,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(31460052)
云南省科技厅-昆明医科大学应用基础研究联合专项(2015FB014)
关键词
DNA宏条形码
中成药
分子鉴定
物种
DNA metabarcoding
Chinese patent medicine
molecular identification
species
作者简介
通讯作者:周静,E-mail:zhoujing_apiaceae@163.com;通讯作者:刘振稳,Tel:(0871)65223161,E-mail:zhenwen1980@hotmail.com;高玉珍,硕士,研究方向为药用植物资源鉴定,E-mail:18487179425@163.com
