摘要
沙门菌在巨噬细胞内的存活是其在宿主内长期定植的主要因素之一,研究利用选择性捕获转录技术(SCOTS)筛选鸡白痢沙门菌S06004株在感染鸡巨噬细胞系HD-11过程中细菌表达的基因。结果显示:鸡白痢沙门菌以MOI值为100∶1感染HD-11细胞1 h后,感染率达11.75%;选取感染1 h时间点作为研究对象,利用SCOTS技术获得了12个鸡白痢沙门菌的转录序列,包括与沙门菌感染相关的毒力基因调控因子inv F;构建了inv F、teh B和tol C基因插入突变株。突变株侵入HD-11的能力明显低于野生株,反映了SCOTS筛选获得的基因在鸡白痢沙门菌感染巨噬细胞时发挥重要的作用,为深入揭示鸡白痢沙门菌的感染机制提供了基础。
Survival in macrophages is one of the main factor for persistent infection of Salmonella in hosts. Selective capture of transcribed sequences(SCOTS)approach was used to identify bacterial genes preferentially expressed by Salmonella Pullorum upon interaction with avian macrophages HD- 11. The result showed that after 1 h infection to HD-11 cells at MOI of 100∶1,the adhesion rate was 11.75%;At this time point,12 transcribed sequences were identified by SCOTS,including regulator gene inv F,required for expression of virulence-related genes involved inSalmonella infection;Three genes(inv F,teh B and tol C)were selected randomly for construction of mutant strains. All the three mutants showed lower infection ability than the wild strain,implying that the sequences identified by SCOTS were involved in Salmonella infection to macrophages. These findings would help to elucidate the pathogenesis of Salmonella.
出处
《中国家禽》
北大核心
2014年第21期17-22,共6页
China Poultry
基金
国家自然科学基金重点项目(31230070)
国家自然科学基金项目(31201905)
江苏省高校优势学科建设项目
