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RNA干扰技术的应用 被引量：2

Application of RNA interference technology
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摘要 RNA干扰 (RNAinterference ,RNAi)是与靶基因序列同源的双链RNA诱导的一种特异性的转录后基因沉默现象。目前 ,RNAi的作用机制已经基本明确 ,并显示了广阔的应用前景。RNAi技术作为新的基因阻断技术已经成功地应用于人类基因组功能的研究 ,同时提供了一条人类抗病毒治疗的新技术。另外 ,借助RNAi技术还有望在将来于基因水平对肿瘤进行治疗。 In 1998, Fire and Mello described a new technology they called RNA interference(RNAi).RNAi is a new technology that is based on the silencing of specific genes by double-stranded RNA(dsRNA).Now investigaters are not only using RNAi to elucidate gene function in mammalian systems,but also develop antiviral therapeutics ,this review provides a general outlook on the application of RNAi.
作者 赵洪波 张嘉宁
机构地区 大连医科大学生物化学教研室
出处 《大连医科大学学报》 CAS 2003年第4期293-295,共3页 Journal of Dalian Medical University
基金 国家自然科学基金项目 序号 396 0 0 0 1 1。
关键词 RNAI SIRNA 基因沉默 RNAi siRNA gene silencing
分类号 Q78 [生物学—分子生物学]
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参考文献15

  • 1[1]Fire A, Xu S,Montgmery MK, et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391:806-811.
  • 2[2]Berstein E,Caudy A,Hammond SM, et al.Role for a bidentate ribonuclease in the initiation step of RNA interference[J].Nature,2001,409:363-366.
  • 3[3]Wassenegger M,Pelissier T.A model for RNA-mediated gene silencing in higher plant [J]. Plant Mol Biol,1998,37:349-362.
  • 4[4]Sayda ME,Jens H,Winfried L, et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in all cultured mammalian cells[J].Nature 2001,411:494-498.
  • 5[5]Nykanen A,Haley B,Zamre PD.ATP requirements and small interfering RNA structure in the RNA interference pathway[J]. Cell,2001,107:309-321.
  • 6[6]Michael T,McManus,Phillip AS.Gene silencing in mammals by small interfering RNAs[J].Nature Reviews Genetics,2002,3:737-747.
  • 7[7]Harborth J,Elbashir SM,Bechert K, et al.Indentification of essential genes in cultured mammalian cells using small interfering RNAs[J].Cell Sci,2001,114:4557-4565.
  • 8[8]Yu JY,DeRuiter SL.,Turner DL.RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells[J].Proc Natl Acad Sci USA, 2002,99:6047-6052.
  • 9[9]Miyagishi M,Taira K.U6 promoter-driven siRNA with four uridine 3'overhangs efficiently surpress targeted gene expression in mammalian cells[J].Nat Biotech,2002,19:497-500.
  • 10[10]McManus MT,Haines BB,Chen J, et al.siRNA-mediated gene silencing in T-cells.J.Immunol (in the press).

同被引文献30

  • 1.[EB/OL].http://www-archbac.u-psud.fr/Graphics/cargese02/sijen1.gif,.
  • 2Brummelkamp TR, Bernards R, Agami R. A system for stableexpression of short interfering RNAs in mammalian cells [J].Science, 2002, 296(5567): 550 - 553.
  • 3Sui G, Soohoo C, Affar EB, et al. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells [ J ].Proc Natl Acad Sci USA, 2002, 99(8): 5515-5520.
  • 4Paul CP, Good PD, Winer I, et al. Effective expression of small interfering RNA in human cells [J]. Nat Biotechnol, 2002, 20(5): 505-508.
  • 5Lee NS, Dohjima T, Bauer G, et al. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells [J]. Nat Biotechnol, 2002, 20(5):500-505.
  • 6GJ汉农编 陈忠斌译.RNAi-基因沉默指南[M].北京:化学工业出版社,2004.118.
  • 7Ambros V. MicroRNAs: Tiny regulator with great potential[J].Cell, 2001, 107(7): 825-826.
  • 8Castanotto D, Li H, Rossi JJ. Functional siRNA expression from transfected PCR products [J]. RNA, 2002, 8(11): 1454-1460.
  • 9Karlas A, Kurth R, Denner J. Inhibition of porcine endogenous retroviruses by RNA interference: increasing the safety of xenotransplantation [ J ]. Virology, 2004, 325(1): 18 - 23.
  • 10Radhakrishnan S, Gordon J, Del Valle L, et al. Intraecellular approach for blocking JC virus gene expression by using RNA interference during viral infection [ J ]. J Virol, 2004, 78(13): 7264-7269.

引证文献2

二级引证文献3

大连医科大学学报
2003年 第4期

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