摘要
探讨卵巢辐射损伤与卵巢切除对大鼠骨组织转移生长因子β1(TGF-β1)与主要的基质蛋白I型胶原(COLI)基因表达的影响。50Gγ剂量γ射线局部照射大鼠卵巢和双侧卵巢切除后应用逆转录-多聚酶链式反应(RT-PCR)法检测大鼠骨组织中TGF-β1与COLI的mRNA表达水平。结果表明,骨组织COLI的mRNA表达水平上升,而TGF-β1mRNA表达减少,卵巢辐射损伤大鼠与卵巢切除大鼠两者结果相似。
To observe the effects of exposure of rat ovary to radiation or OVX on the expression of TGF-β1 and COLI in the rat bone. The mRNA levels of TGF-β1 and COLI in rat tibiae were measured with RT-PCR after the rat ovaries were irradiated by 50Gy of 137Cs γ-rays or OVX. For both the radiation group and the OVX group, the COLI mRNA level in the rat bone increased, whereas the TGF-β1 decreased. Irradiation of ovary and OVX affect the expression of COLI and TGF-β1 mRNA in bone probably in a similar way which is related to estrogen decrease.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2003年第4期280-283,共4页
Journal of Radiation Research and Radiation Processing
基金
国家教育部博士学科点基金(96026517)资助
