摘要
利用多种生物信息学软件对本实验室分离的1株T4类多价烈性噬菌体Bp7尾丝蛋白gp37基因与其他5株T4类噬菌体gp37基因进行密码子特征的分析比较。结果显示:Bp7与其他5株T4类噬菌体gp37基因密码子使用模式基本一致;Bp7尾丝蛋白gp37基因密码子偏好性较低,且偏爱使用以A或U为结尾的密码子;除AUG(Met)和JGG(Trp)外,确定了17种高频密码子;6株菌体gp37之间密码子偏好性比较发现,Bp7与JS98密码子使用最相近,与T4和T4T相对较远;gp37基因密码子的使用模式可能受中性突变和选择压力的共同作用。gp37基因密码子的分析结果将为Bp7尾丝蛋白的基因改造,以进一步拓宽其裂解谱及提高表达水平提供理论指导。
Polyvalent coliphage Bp7 was isolated and identified as T4-like phage,the codon usage in gene gp37 was analyzed by various bioinformatics softwares.The results were showed as follows:The patterns of codon usage in gene gp37 of phage Bp7 and other 5 T4-like phages were almost similar.In gene gp37 of phage Bp7,the codon bias was a little low,and gp37 seemed like using A or U ending codon;17 kinds of high-frequency codons were identified except AUG(Met)and UGG(Trp).The codon bias comparison of gp37 among phage Bp7 and other 5 phages showed that codon usage in Bp7 was most similar to JS98,a little far from T4 and T4 T relatively.The codon usage patterns of gp37 may be influenced not only by selection pressure,but also by neutral mutation.The results of anlysis of gp37 codons will benefit to the modification of tail fiber protein of Bp7 and further broaden the host range of phage Bp7,and improve the level of gp37 expression as well.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2014年第8期1330-1335,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
山东省自然科学基金(ZR2009DM009
ZR2013CQ024)
