摘要
【背景】目前,海水养殖业中主要利用抗生素来防治哈维氏弧菌等病原菌,但抗生素的长期使用或滥用会对环境和人体健康带来危害,因此既环保又有效的生物防治方法具有广阔的应用前景。【目的】从海水产品共生微生物中筛选具有抗菌活性的菌株,对活性菌株进行鉴定并确定其合成抗菌活性物质的培养条件。【方法】利用沙氏和2216E培养基,以稀释涂布平板法从海水养殖动物中分离真菌和细菌;利用牛津杯法测定微生物发酵液抗水产病原哈维氏弧菌的活性;通过菌株的培养特征、形态特征和ITS序列分析对抗菌活性菌株进行鉴定;通过筛选发酵培养基的种类及盐度确定培养条件。【结果】从海蚌、白虾、海蛎子等9种样品中分离出微生物52株,其中真菌30株、细菌22株;筛选得到2株具有抗哈维氏弧菌活性的真菌菌株;其中一株活性菌株HLZ-3被鉴定为塔宾曲霉;菌株HLZ-3合成抗菌活性物质的培养条件为4%NaCl的大米培养基,28°C静置培养2周。【结论】实验结果为进一步分离纯化菌株HLZ-3所产抗菌活性次生代谢产物提供了基础。
[Background]At present,antibiotics are mainly used to control pathogenic bacteria such as Vibrio harveyi in seawater breeding industry.However,the long-term application or abuse of antibiotics may be adverse to the environment protection and human health.Therefore,biological control methods that are both environmentally friendly and effective have broad application prospects.[Objective]Strains with antibacterial activity were screened from the symbiotic microorganisms of seawater products.The active strains were identified and the culture conditions for the synthesis of antibacterial active substances were determined.[Methods]Fungi and bacteria were isolated from mariculture animals by the dilution and spreading plate method using the culture medium of Sabouraud and 2216 E medium.The antibacterial activity of microbial fermentation broths against V.harveyi was determined by Oxford cup method.The active strain was identified by the morphological characteristics and internal transcribed spacer(ITS)analysis.Culture conditions were determined by screening the type and salinity of fermentation medium.[Results]A total of 52 microbial strains were isolated from 9 kinds of samples,among them,30 strains belong to fungi and 22 strains belong to bacteria.Two fungal strains with antibacterial activity against V.harveyi were obtained.One active strain,HLZ-3,was identified as Aspergillus tubingensi.The culture conditions of HLZ-3 for the synthesis of antibacterial active substances was 4%NaCl in rice culture medium,and stationary incubated at 28°C for 2 weeks.[Conclusion]The results provide a basis for further isolation and purification of antibacterial secondary metabolites produced by the strain HLZ-3.
作者
陈慧
曹曦
王鑫彤
张菲
王乐
郭雷
CHEN Hui;CAO Xi;WANG Xin-Tong;ZHANG Fei;WANG Le;GUO Lei(Jiangsu Key Laboratory of Marine Bioresources and Environment,School of Marine Life and Fisheries,Jiangsu Ocean University,Lianyungang,Jiangsu 222005,China;Marine Resources Development Institute of Jiangsu,Lianyungang,Jiangsu 222005,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2019年第10期2475-2481,共7页
Microbiology China
基金
江苏省自然科学基金(BK20151283)
连云港市科学计划项目(CG1612)
江苏省研究生实践创新计划项目(SJCX19_0955)
连云港市521人才工程项目(KKC17001)~~
关键词
共生微生物
抗菌活性
菌种鉴定
培养条件
Symbiotic microorganisms
Antibacterial activity
Strain identification
Culture conditions
作者简介
通信作者:郭雷,E-mail:leiguoo@sina.com
