摘要
目的 :探讨犬颅脑火器伤后脑组织内脑源性神经生长因子 (Brain derivedneurotrophicfactor,BDNF)的变化规律及其对颅脑火器创伤神经元的保护作用 .方法 :建立犬颅脑枪弹伤模型 ,制备不同部位不同时间点脑组织切片 ,免疫组化法染色 ,检测BDNF的表达 .与设立的对照组相比较 ,并进行组间比较 .结果 :损伤组脑组织BDNF含量明显高于对照组 ,动物的伤情相对愈重 ,表达愈明显 .在 2～ 4h为表达高峰期 ,不同组织间亦存在明显差异 (P <0 .0 1 ) ,以海马及挫伤区含量高 .结论 :颅脑枪弹伤后不同部位脑组织BDNF随时间的变化出现不同表达 ,提示BDNF在颅脑枪弹伤的早期修复中发挥作用 .
AIM: To observe the expression of BDNF in cerebral cortex,hippocampus CA2 and brain stem in canines with cranicerebral gunshot wounds. METHODS: Twenty canines were divided into normal control and injury groups. Using the model of gunshot injured canine cerebrum made by the bullets of German made small caliber rifles, BDNF positive neurons were observed in the contusion trajectory area, the concussion trajectory area, hippocampus and brain stem at 30 min, 1, 2, 4, 6, 8 and 12 h after trauma by immunohistochemical method. The results of the control group and injury group were compared. RESULTS: The expression of BDNF in the injury group was obviously higher than that of the control group. The expression appeared 2 ～ 4 hours after trauma ( P <0.01). CONCLUSION: BDNF plays a positive role in repair of brain injury.
出处
《第四军医大学学报》
北大核心
2003年第22期2063-2065,共3页
Journal of the Fourth Military Medical University
基金
全军"九五"医学科研基金重点资助项目 (99 Z 1 4 6)
