摘要
目的 :探讨转化生长因子 - β1,2 (TGF - β1和TGF - β2 )与α -平滑肌肌动蛋白 (α -ASMA)在胎儿和成人皮肤中的分布和表达变化规律。方法 :2 0例被测标本中包括不同胎龄的胎儿皮肤 15例和成人皮肤 5例。用免疫组化方法和病理技术确定这 3种蛋白在不同发育阶段皮肤中的定位和表达量。结果 :在妊娠早期的胎儿皮肤中 ,TGF - β1,2 和α-ASMA呈弱阳性表达 ,随着胎儿生长发育 ,这 3种蛋白的阳性细胞率逐渐增大 ;在妊娠晚期胎儿和成人皮肤中这 3种蛋白的表达量明显多于妊娠早期胎儿皮肤 (P <0 0 1)。TGF - β1,2 的阳性信号主要存在于表皮细胞 ,内皮细胞和部分成纤维细胞中 ,α -ASMA蛋白定位于肌成纤维细胞和汗腺上皮细胞内。结论 :TGF - β1和TGF - β2 可能在皮肤的发生、结构和功能的维持以及皮肤损伤后的修复中起重要作用。
AIM: To explore the localization and expression of transfo rming grow th factor-β 1,2 (TGF-β 1,2 ) and alpha-smooth muscle actin (α- ASMA) in fetal a nd adult skins. METHODS: Skins of 15 cases of fetuses with different gestational ages and 5 cases of adults were taken, embedded with paraffin wax, and sectione d. Immunohistochemistry method and pathological method were used to detect the e xpression intensity and distribution of TGF-β 1,2 and α-ASMA. RESULTS: Positive immunohistochemical signals of TGF-β 1,2 and α-A SMA were found in fetal and adult skins. In skins derived from young fet us, the positive signals of these three proteins were very weak. Along with the incr ement in gestational age, the positive cellular rates of TGF-β 1,2 and α- ASMA were elevated pro gressively. In elder fetal and adult skins, TGF-β 1,2 were mostly distributed i n epidermal cells, endothelial cells and some fibroblasts, while α-ASMA was mainly located in myofibroblasts and sweat gland epithelial cells. CONCLUSION: The endo genous TGF-β 1,2 might be involved in the cutaneous development at embryoni c stage, in the cutaneous structure maintenance at adult stage, and in the wound healing af ter injury.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2003年第10期1297-1299,T001,共4页
Chinese Journal of Pathophysiology
基金
国家重大基础研究规划资助项目 (No.G1999054204 )
国家杰出青年科学基金资助项目 (No.39525024 )
国家自然科学基金重点项目(No.30230370)
