摘要
目前在人胚胎多能干细胞的研究中存在材料来源不足、培养体系复杂和自发性分化的问题。这 3个基本问题限制了人胚胎多能干细胞的基础和临床应用研究。要建立新的胚胎多能干细胞系应利用细胞核移植和细胞质重新编程功能建立新的途径 ,并探讨其中的机制和可能的关键因子 ;干细胞培养时主要进行简化培养体系和抑制细胞分化的研究 ;对干细胞分化进行临床应用研究时 。
Presently, there are 3 problems in the study of human embryonic pluripotent stem cells: short of materials, complicacy of culture system and spontaneous differentiation. Both basic research and clinical application of human embryonic pluripotent stem cells were hindered by these 3 basic problems. New embryonic pluripotent stem cells should be established by combining nucleus transfer and plasma reprograming, and their possible mechanisms and key factors need further study. The culture system should be simplified and cell differentiation must be inhibited when pluripotent stem cell is cultured. When pluripotent stem cells are induced to differentiation, combination of several methods should be used to induce and select specific cell lineages, and the morphology and function of the target cell must be evaluated.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2003年第9期933-936,共4页
Academic Journal of Second Military Medical University
基金
国家杰出青年科学基金 ( 3 982 5 116)
海外青年学者合作研究基金 ( 3 992 80 0 9)
