摘要
目的 研究重组人干扰素α2b、α1b、β1b和ω1b对严重急性呼吸综合征 (SARS)病毒在细胞培养上的抑制作用。方法 采用细胞病变抑制法在人横纹肌瘤 (Rda)细胞系培养上测定干扰素的抗SARS病毒活性。结果 高度纯化的重组人干扰素α2b、α1b、β1b和ω1b在Rda细胞培养上抑制SARS病毒产生 5 0 %细胞病变 (CPE)的最小活性单位各为 (16 0 5± 12 9 5 )IU ml、(14 9 0± 71 7)IU ml、(6 9 5± 6 1 5 )IU ml、(87 3± 4 7 1)IU ml或各为 (0 6± 0 5 )ng ml、(10 6± 5 1)ng ml、(3 5± 3 1)ng ml、(0 9± 0 5 )ng ml。结论 本研究中所用的重组人干扰素在细胞培养上均具有较好的抑制SARS病毒的活性。
Objective To Study the anti SARS virus activities of different recombinant human interferons on the cell culture system. Methods Anti SARS virus activities of interferons were determined by using CPE inhibition test in human skeletal muscle sarcoma (Rda)cell culture. Results The average minimum amount of interferon alpha 2b, alpha 1b, beta 1b or omega 1b to inhibit 50% CPE in Rda cell culture was (160.5±129.5) IU/ml, (149.0±71.7) IU/ml, (69.5±61.5) IU/ml, (87.3±47.1) IU/ml, respectiveily or (0.6±0.5) ng/ml, (10.6±5.1) ng/ml, (3.5±3.1) ng/ml, (0.9±0.5) ng/ml, respectively. Conclusion All the tested recombinant interferons showed anti SARS virus activities on the Rda cell culture with different sensitivities.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2003年第3期205-208,共4页
Chinese Journal of Experimental and Clinical Virology
基金
"十五" 863计划资金资助项目 (2003AA2 0 82 0 6
2 0 0 3AA2 0 82 0 9)
关键词
重组人干扰素
细胞培养
SARS病毒
临床应用
免疫机能
Inteferon type Ⅰ, Recombinant
Severe acute respiratory syndrome
CoronaviridaeDevelopment of the cDNA chip for SARS virus and a primary study on the possible molecular mechanism of interferon α2b inhibiting the SARS virus replication $$$$
