摘要
在合成一种新的吡咯衍生物DNA-3-吡咯甲基-四聚乙二醇单醚的基础上,探索了恒电位法在铂盘电极上的电化学聚合,利用扫描电子显微镜(SEM)、X-射线能量散射谱(EDX)等对聚合膜的表面形貌和元素组成进行了测试分析。探索了利用亚甲基蓝(MB)进行DNA芯片杂交检测的可行性。实验结果证明,MB可以用作无标记的DNA杂交检测,同时证明了基于恒电位聚合制作的DNA芯片是有检测功能的。
A novel Poly - derivative 3 - pyrrole - DNA with 3 - pyrrole derivatitv was successfully electrochemical deposited on the electrode surface at 1.1 V. The electrode modified with 3 -pyrrole -DNA was characterized with Scanning Electro Microscopy (SEM) and Energy Dispersive X -ray Analysis (EDX). The results showed that the derivatives have been successfully electropolymerized on the electrode and the DNA has been immobilized. The hybridization on the 3 -pyrrole -DNA polymer modified electrode have been investigated with methylene blue (MB) as an indicator, thus the function of 3 - pyrrole - DNA modified electrode was qualitatively analyzed.
出处
《化学传感器》
CAS
2003年第3期7-13,共7页
Chemical Sensors
基金
中科院知识创新项目子课题(BL200107)
