摘要
以9-芴乙酸(fluorene-9-aceticacid,FLU)为原料,经活性酯法合成完全抗原FLU-牛血清白蛋白(bovine serum albumin,BSA)和FLU-卵清白蛋白(ovalbumin,OVA),偶联产物通过非变性聚丙烯酰胺凝胶电泳及紫外扫描法鉴定。使用FLU-BSA足垫免疫BALB/c小鼠获得多克隆抗体,间接酶联免疫吸附实验(enzyme linked immunosorbent assay,ELISA)测得多克隆抗体效价为51 200,间接竞争ELISA方法测得芴多克隆抗体的特异性,与荧蒽的交叉反应率为12%,与其他14种多环芳烃交叉反应不明显。
Fluorene-9-acetic acid(FLU) was chosen as the hapten and was coupled with bovine serum albumin(BSA) and ovalbumin(OVA) respectively to prepare complete antigens by active ester method. The complete antigens were characterized by native polyacrylamide gel electrophoresis and ultraviolet spectrum. FLU-BSA was used to obtain polyclonal antibody through footpad injection of BALB/c mice. The titer was 51 200 as measured by indirect enzyme linked immunosorbent assay(ELISA), and the cross-reactivity with fluoranthene was measured by indirect competitive ELISA to be 12% while having no obvious cross-reaction with 14 other kinds of polycyclic aromatic hydrocarbons(PAHs). This ELISA method for fluorene can lay the foundation for further experimental research.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第9期108-112,共5页
Food Science
基金
吉林省科技发展计划项目(20140101012JC)
国家自然科学基金面上项目(61171022
60971011
30771657)
关键词
9-芴乙酸
完全抗原
多克隆抗体
酶联免疫吸附实验
fluorene-9-acetic acid
complete antigen
polyclonal antibody
enzyme linked immunosorbent assay(ELISA)
