摘要
长双歧杆菌BBMN68分离自广西巴马长寿老人粪便,已证明其具有双歧杆菌普遍的生理功能。为解决其不耐酸、不耐氧的缺点,本实验采用微胶囊包埋技术,以海藻酸钠、乳清蛋白以及VE为壁材,在氮气体系中采用挤压法包埋该双歧杆菌活菌体制备湿微胶囊和冻干微胶囊。结果表明:海藻酸钠、乳清蛋白和VE质量分数分别为2%、3%和10%条件下制备的湿微胶囊在酸奶中保存21 d仍能保持106 CFU/g以上的活菌数,耐胃酸实验2.0 h时活菌数仍高达3.16×107 CFU/g,肠溶实验中微胶囊在2.0 h实现完全崩解;海藻酸钠和乳清蛋白质量分数为2%和3%,氮气环境下制备的冻干微胶囊通过恒温加速实验测定可在室温条件下贮藏长达75 d。通过微胶囊包埋技术较好地解决了长双歧杆菌BBMN68不耐酸、不耐氧的缺点,同时也提供一种新型有效的补充肠道益生菌的形式。
Bifidobacterium longum subsp. longum BBMN68 is a new strain isolated from the feces of a centenarian living in an area of Bama, Guangxi, China, known for the longevity of its residents. BBMN68 has general health benefits of Bifidobacterium, but it is sensitive to oxygen and low p H. In order to resolve the shortcomings, microcapsules were prepared by using sodium alginate, whey protein and vitamin E as wall materials, an extrusion method was used to protect BBMN68 cells in the nitrogen environment, and freeze-dried microcapsules were produced simultaneously. The results showed that the wet microcapsules prepared under the conditions of sodium alginate 2%, whey protein 3%, vitamin E 10%(wt%) could maintain the viable cells of 106 CFU/g after 21 d storage in yogurt, and the viable cells were up to 3.16×107 CFU/g after 2 h in stimulated gastric fluid. The wet microcapsules were disintegrated completely in stimulated intestinal fluid. The freeze-dried microcapsules prepared under the conditions of sodium alginate 2% and whey protein 3% in the nitrogen environment were stable for up to 75 days at room temperature through isothermal accelerated test. Therefore, the microencapsulation technology can resolve well the above-mentioned shortcomings of BBMN68, while providing a new and effective form for supplementing probiotics.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第9期71-77,共7页
Food Science
基金
国家自然科学基金面上项目(31171771)
"十二五"国家科技支撑计划项目(2011BAD23B04)
关键词
长双歧杆菌BBMN68
双乳化
微胶囊
真空冷冻干燥
模拟胃肠实验
Bifidobacterium longum BBMN68
double emulsification
microcapsules
vacuum freeze-drying
stimulated gastrointestinal experiment
