摘要
以禾谷镰刀菌Fusarium graminearum大型分生孢子定量接种大米培养物,制备毒素粗提液,采用硅胶柱一步洗脱分离、分段收集可同时获得脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)和乙酰化脱氧雪腐镰刀菌烯醇(acetyldeoxynivalenol,AcDON),采用一步结晶法即可分别制备获得毒素纯品,并经红外光谱法、液相色谱-质谱联用技术及核磁共振氢谱等分析确证,液相色谱分析表明两种毒素纯度均大于98%。该培养提取及分离制备方法改变了常规多步骤繁琐过程,提供了一种切实有效的、大量制备纯化DON及乙酰化DON毒素的简便方法。
Fusarium graminearum macroconidia were quantitatively inoculated in rice culture, and the crude extracts of the toxins were prepared and subjected to silica gel column chromatography. Deoxynivalenol(DON) and acetyldeoxynivalenol(AcDON) isolates were monitored by thin-layer chromatography and the fractions rich in these toxins were pooled and concentrated, respectively. Both fractions yielded a crystalline material through a simple crystallization step. The two compounds were identified and confirmed by infrared spectroscopy, liquid chromatography-mass spectrometry and1 NMR analysis in comparison to the reference standards or the published data. The liquid chromatography analysis showed that the purity of the two toxins were both greater than 98% This method developed here has overcome the disadvantages of the conventional cumbersome multi-step procedure, and provided a simplified and efficient strategy suitable for large-scale production and purification of the mycotoxin DON and its acetylated products AcDON from Fusarium graminearum rice cultures.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第3期132-136,共5页
Food Science
基金
“十二五”国家科技支撑计划项目(2011BAK10B07-1)
