摘要
研究H2SO4降解法制备不同分子质量岩藻聚糖硫酸酯的降解条件及其产物的化学组成。依次采用DEAE-52纤维素离子交换层析法、H2SO4降解法、Sephacryl S-200凝胶色谱,制备不同分子质量的岩藻聚糖硫酸酯。运用高效凝胶色谱测定其相对分子质量,并借助衍生化气相色谱和红外光谱对其单糖的组成和结构进行解析。结果表明:在60℃下,含有1%岩藻聚糖硫酸酯的0.05 mol/L H2SO4溶液反应4 h和8 h时后,可以有效可控地将围氏马尾藻岩藻聚糖硫酸酯降解,经纯化后得到MMWF1、MMWF2、LMWF1、LMWF2四组分,其分子质量分别为16.32、15.49、7.65和7.21 k D,总糖含量分别为57.89%、55.45%、56.18%和52.64%,硫酸基含量分别为18.89%、25.11%、14.42%和19.76%。另外,4组分均由鼠李糖、岩藻糖、木糖、甘露糖、葡萄糖和半乳糖组成,但各组分的单糖含量不同,红外光谱测定结果显示其均含有多糖的特征吸收峰和硫酸基的吸收峰。
To investge the coditons o sulfuric acid egradation to prepare different molecular weithg fucoidan and to analyze the chemical compostions of fucoidan. Fucoidan was prepared from crude S. wightii by DEAD-52 cellulose iron exchange chromatography,sulfuric acid degradation and Sephacryl S-200 gel chrlmotography in sequence. Gel chromatography was used to determine the molecular weight,derivative gas chromatography and infrared spectroscopy were applied to analyze the monosaccharide compositions and their structures. The results showed that four different molecular weight fractions( MMWF1,MMWF2,LMWF1 and LMWF2) were obtained. The degradation conditions were 1% fucoidan in 0. 05 mol/L sulfuric acid solution reacted for 4 h and 8 h respectively at 60 ℃. With the average molecular weight of 16. 32,15. 49,7. 65 and 7. 21 k Da respectively,their total sugar content were 57. 89%,55. 45%,56. 18% and 52. 64% and the sulfate content were 18. 89%,25. 11%,14. 42% and 19. 76% respectively. All four fractions contained rhamnose,fucose,xylose,mannose,glucose and galactose,but their monosaccharide contents were different. Infrared spectroscopy verified that four fractions allhad polysaccharide and sulfate absorption peaks.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2015年第6期70-75,共6页
Food and Fermentation Industries
基金
广东省科技计划项目<马尾藻食品加工技术研究及开发>(编号:2011B020310010)
关键词
围氏马尾藻
岩藻聚糖硫酸酯
酸降解
分级纯化
化学组成
Sargassum wightii
fucoidan
sulfate acid degradation
classification and purification
chemical composition
