摘要
目的研究草甘膦对转基因大豆GTS40-3-2生理特性的影响。方法实验室盆栽种植转基因大豆GTS40-3-2,在GTS40-3-2第三复叶期喷施不同浓度的草甘膦处理,24h后取叶片分别进行生理指标检测。结果叶绿素含量对照组最高,且随草甘膦浓度的增加而降低;不同浓度的草甘膦处理叶片的相对电导率(electrolyticleakage,EL)和丙二醛(malonaldehyde,MDA)含量与对照组相比无显著差异(P>0.05);与对照组相比较,草甘膦喷施导致过氧化氢酶(catalase,CAT)活性下降,随草甘膦浓度的增加,CAT活性无显著差异(P>0.05),超氧化物歧化酶(superoxide dismutase, SOD)、过氧化物酶(peroxidase, POD)活性升高。POD活性受草甘膦影响较大, SOD活性受草甘膦影响较小。结论喷施草甘膦对GTS 40-3-2生理特性存在一定的影响,应注意日常施用量。
Objective To investigate the effect of glyphosate on the physiological characteristics of transgenic soybeans GTS 40-3-2.Methods The transgenic soybean GTS 40-3-2 was planted in the laboratory pot,and different concentrations of glyphosate were sprayed at V3 stage of GTS 40-3-2.After 24 h,the leaves were taken for physiological index detection.Results The chlorophyll content of the control group was the highest and decreased with the increase of glyphosate concentration.The relative electrical conductivity(EL)and malonaldehyde(MDA)content of the leaves treated with different concentrations of glyphosate were not significantly different from those of the control group(P>0.05).Compared with the control group,glyphosate spray resulted in decreased catalase(CAT)activity.With the increase of glyphosate concentration,there was no significant difference in CAT activity(P>0.05),and the activities of superoxide dismutase(SOD)and peroxidase(POD)increased.The POD activity was greatly affected by glyphosate,while the SOD activity was less affected by glyphosate.Conclusion Spraying glyphosate has a certain effect on the physiological characteristics of GTS 40-3-2,and should pay attention to the daily application rate.
作者
常丽娟
刘文娟
张富丽
王东
宋君
CHANG Li-Juan;LIU Wen-Juan;ZHANG Fu-Li;WANG Dong;SONG Jun(Analysis and Test Center,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China)
出处
《食品安全质量检测学报》
CAS
2019年第16期5453-5458,共6页
Journal of Food Safety and Quality
基金
省财政基因工程青年基金项目(2018QNJJ-023)~~
关键词
转基因大豆
草甘膦
生理特性
genetically modified soybean
glyphosate
physiological characteristics
作者简介
通讯作者:常丽娟,硕士,助理研究员,主要研究斱向转基因生物安全监管。E-mail:277989595@qq.com
