摘要
目的研究原型脱氧雪腐镰刀菌烯醇(deoxynivalenol, DON)及其修饰型的产生规律。方法以小麦籽粒为培养材料,辐照灭菌后接种禾谷镰刀菌,并分别在不同温度(10、20、30℃)和水活度(0.95、0.98 a_w)下培养不同时间(7、14、21、28、35d)后,运用超高效液相色谱-串联质谱检测DON,3-乙酰化脱氧雪腐镰刀菌烯醇(3-acetyl-deoxynivalenol,3-ADON),15-乙酰化脱氧雪腐镰刀菌烯醇(15-acetyl-deoxynivalenol,15-ADON)和脱氧雪腐镰刀菌烯醇-3-葡萄糖苷(deoxynivalenol-3-glucoside,D3G)的产量。结果在所有培养条件下,原型DON的产量均随时间延长而增加;并于0.98 a_w, 20℃下的第35 d达到最高值100490.0μg/kg;不同培养条件下,修饰型DON的生成情况各不相同,3-ADON、15-ADON和D3G分别于0.98a_w,20℃下的第14、28和35 d达到各自产量最高值(3-ADON:7583.5μg/kg; 15-ADON:592.0μg/kg; D3G:6806.4μg/kg)。此外, 10℃下,D3G/DON比值随时间延长先增高后降低,而20、30℃下D3G/DON比值随时间延长呈指数下降(r^2>0.90)。结论小麦籽粒中原型和修饰型DON的最适产生条件均为0.98 a_w, 20℃。本研究可为DON及其修饰型的生物合成、日常监管、污染防控等提供理论基础和科学依据。
Objective To investigate the production mechanisms of free and modified forms of deoxynivalenol(DON).Methods Irradiated wheat grains were inoculated artificially with Fusarium graminearum and then incubated at different temperatures(10,20,30℃)and water activities(0.95,0.98 a_w).After incubation of 7,14,21,28 and 35 days,the production of DON,its modified forms of 3-acetyl-deoxynivalenol(3-ADON),15-acetyl-deoxynivalenol(15-ADON)and DON-3-glucoside(D3 G),were quantified by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Results DON contents increased gradually in time dependent manner in all conditions,and the highest yield was 100490.0μg/kg on day 35 at 20℃and 0.98 a_w.However,the highest yields of 3-ADON,15-ADON and D3 G were observed on day 24,18 and 35 at 20℃and 0.98 a_w(3-ADON:7583.5μg/kg;15-ADON:592.0μg/kg;D3 G:6806.4μg/kg),respectively.In addition,the ratio of D3 G/DON increased firstly and then decreased at 10℃,and declined gradually at 20℃and 30℃,which could be well described by exponential model(r^2>0.90).Conclusion The optimum temperature for free and modified forms of DON production was 20℃and the optimum water activity was 0.98 a_w.Our results could provide theoretical base and scientific foundation for biosynthesis,routine supervision,prevention and control of free and modified DON.
作者
范楷
刘星
郭文博
张志岐
孟佳佳
杜颖
聂冬霞
FAN Kai;LIU Xing;GUO Wen-Bo;ZHANG Zhi-Qi;MENG Jia-Jia;DU Ying;NIE Dong-Xia(Institute for Agro-food Standards and Testing Technology,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)
出处
《食品安全质量检测学报》
CAS
2019年第9期2545-2554,共10页
Journal of Food Safety and Quality
基金
上海市科技兴农重点攻关项目(G20150632)~~
作者简介
通讯作者:聂冬霞,副研究员,主要研究方向为农产品质量安全。E-mail:niedongxia@163.com;范楷,助理研究员,主要研究方向为农产品质量安全。E-mail:fankai1983@foxmail.com
