摘要
目的建立快速检测传染性造血器官坏死病病毒(IHNV)的间接酶联免疫法。方法以传染性造血器官坏死病毒G蛋白(IHNV-G)为抗原,免疫实验白兔,制备兔抗IHNV免疫血清,通过ELISA对兔抗血清进行效价及特异性评估,并通过PCR方法进行验证。结果应用间接ELISA技术,检测到抗血清对IHNV-G和IHNV的效价分别达到1:32000和1:16000,与其他对照病毒无交叉反应。与PCR检测结果符合率达到98%。结论本文建立的间接酶联免疫法方法具有良好的灵敏性和特异性,为IHNV的检测提供了快捷、高效的技术手段。
Objective To establish an indirect enzyme-linked immune assays(ELISA)for the identification of infectious hematopoietic necrosis virus(IHNV).Methods An experimental rabbit was immunized to produce anti-IHNV serum with IHNV-G protein.To assess the titer and specificity of the anti-serum,the ELISA test was used.Further,PCR was used to verify the above ELISA experimental result.Results The titer of anti-serum of IHNV-G and IHNV reached to 1:32000 and 1:16000,respectively,moreover,the cross-reaction with other virus was 0%,and ELISA results was with 98% accordance rate with the method of PCR.Conclusion The indirect-ELISA of this research has a good specificity and sensitivity,and it is suitable for rapid detection and epidemiological investigation of IHNV infection in trout.
出处
《食品安全质量检测学报》
CAS
2014年第8期2327-2331,共5页
Journal of Food Safety and Quality
基金
辽宁省海洋与渔业厅研究基金项目(2011014)~~
