摘要
目的:探究不同浓度镉胁迫对乌龟脂质过氧化损伤的影响。方法:健康实验龟随机分为对照组、7.5 mg/kg组、15mg/kg组和30 mg/kg组。7.5 mg/kg组、15 mg/kg组和30 mg/kg组分别腹腔注射7.5 mg/kg、15 mg/kg、30 mg/kg灭菌的氯化镉溶液染毒,对照组腹腔注射等容量的生理盐水。结果:与对照组比较,7.5 mg/kg组、15 mg/kg组和30 mg/kg组乌龟血浆中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和谷胱甘肽(GSH)含量在染毒后1 w、2 w、3 w均不同程度降低,而丙二醛(MDA)含量有不同程度的升高。结论:实验龟腹腔注射7.5 mg/kg、15 mg/kg、30 mg/kg氯化镉溶液引起了氧化损伤。
Objective:The study investigated the lipid peroxidation lesion of freshwater turtle Chinemys reevesii exposed to different concentration cadmium. Methods:The experimental turtles were randomly divided into control group,7.5 mg/kg group,15 mg/kg group and 30 mg/kg group,and were exposed to 0 mg/kg,7.5 mg/kg,15 mg/kg,30 mg/kg sterile cadmium chloride and normal saline separately by intraperitoneal injection. Results:Compared with the control group,SOD,CAT activities and content of GSH in plasma of the 7.5 mg/kg,15 mg/kg,30 mg/kg groups were decreased in 1 week,2 weeks and 3 weeks. However,content of MDA was increased. Conclusion:Experiment turtles exposed to cadmium at the concentration of 7.5 mg/kg,15 mg/kg,30 mg/kg were led to oxidative Lesion.
作者
霍俊凤
董爱国
Huo Junfeng;Dong Aiguo(Shanxi University of Chinese Medicine,Jinzhong Shanxi 030619)
出处
《山西中医学院学报》
2019年第1期17-19,共3页
Journal of Shanxi College of Traditional Chinese Medicine
基金
山西省中医药管理局基金(2016ZYYC05)
山西中医药大学博士基金(2015BK13)
山西省应用基础研究项目(20181D121229)
关键词
脂质过氧化损伤
镉胁迫
乌龟
lipid peroxidation lesion
cadmium stress
turtles
作者简介
霍俊凤,高级实验师,博士,E-mail:huojunfeng1995@126.com;通讯作者:董爱国,博士,副教授,E-mail:dongaiguo2000@126.com
