摘要
目的 观察B7- 1基因转染对小鼠前胃癌细胞 (MFC)增殖活性的影响。方法 通过脂质体介导将B7- 1基因转染MFC细胞 ,G4 18筛选阳性克隆后 ,用MTT法和流式细胞仪 (FCM )分别测定转染然细胞增殖活性的变化 ,并以空载体 pcDNA3转染MFC细胞作为对照。 结果 转染B7- 1基因的MFC细胞增殖活性受到抑制。结论 转染B7- 1基因的MFC细胞增殖活性受到抑制 ,提示B7-
objective To investigate the effect of B7-1 gene transfer on proliferation chactivity of MFC cells. Methods MFC was transferred by B7-1 gene through the liposomes induced. The positive clone was chosen by G418, and then the proliferation was assayed by MTT and FCM. The result was compared with the resuilt of MFC trans ferred by blank-carrier NDA3.Results The proliferation of MFC that were transferred by B7-1 was inhibited. Conclusion B7-1 gene-transferred MFC cells can effectively inhibit the proliferation of MFC cells.
出处
《湖北民族学院学报（医学版）》
2003年第2期1-3,共3页
Journal of Hubei Minzu University(Medical Edition)
