摘要
从大连海岸的海泥中分离到 4株海洋光合细菌 :菌株C4 10、菌株DS2、菌株E3 和E4,它们都能在厌氧光照下营光异养生长 ,菌株C4 10还能够利用还原性硫化物营光自养生长 .依菌对NaCl的需求 ,菌株C4 10、DS2归属于嗜盐光合细菌 ,菌株E3 和E4属于耐盐光合细菌 .根据形态和培养特征、生理生化特征、光合作用内膜结构、泛醌组成、(G +C)的摩尔百分比等指标 ,菌株C4 10鉴定为Rhodovulumsulfidophilus (嗜硫小红卵菌 )、菌株DS2鉴定为Rhodobiummarinum (海红菌 )、菌株E3 和E4鉴定为Rhodobacterazotoformans.4株菌的营养成分分析表明 ,它们的细胞的最大生长量为 4× 10 9mL-1.粗蛋白含量占细胞干重的 5 5 %左右 ,菌株DS2高达 6 4 .2 % .4菌株所含氨基酸种类齐全 ,特别具备人和动物所必需的氨基酸 .4株菌均含有辅酶Q10和类胡萝卜素 .其中菌株C4 10的类葫萝卜素含量最高 .图 1表 6参
Four strains of marine photosynthesis bacteria were isolated from seawater and the sediments of Dalian coast. All four strains were motile. They required one or more kinds of growth factors. The isolates were able to grow anaerobically in light or aerobically in dark. Strain C410, E 3 and E 4 contained vesicular intracytoplasmic membranes unlike strain DS2, which contained a lamellar one. Because salt was necessary for the growth of strains C410 and DS2, they belonged to halophilic purple nonsulfur bacteria. Strains E 3 and E 4 belonged to salt tolerant purple nonsulfur bacteria. Strain C410 grew well using sulfide or thiosulfate as electron donors for phototrophic growth. They were identified as Rhodovulum sulfidophilus (strain C410), Rhodobium marinum (strain DS2), and Rhodobacter azotoformans (strains E 3 and E 4). Their cell yields, protein contents, amino acid compositions, and contents of carotenoids were analyzed under the optimum conditions. All of the most cell yields of the four strains were more than 4×10 9/mL -1 .Their protein contents of dry weights were about 55% (for strain DS2 it was 64.2 %). They contained all kinds of essential amino acids, and also contained coenzyme Q10 and carotenoid. The carotenoid content of strain E 3 was the most among the four strains. Fig 1, Tab 6, Ref 14
出处
《应用与环境生物学报》
CAS
CSCD
2003年第3期298-301,共4页
Chinese Journal of Applied and Environmental Biology
基金
海洋 8 63项目 (2 0 0 1AA62 30 4 0 )资助~~
关键词
嗜盐光合细菌
分离
鉴定
营养成分
marine photosynthesis bacteria
halophilic purple nonsulfur bacteria
isolation and identification
nutrient composition
