摘要
目的 :尝试在兔眼角膜上建立有效的大泡性角膜病变 (bullouskeratopathy ,以下简称BK)模型 ,为今后研究该病的损害机制和治疗方法奠定基础。方法 :将 11只成年新西兰大白兔 ,分为实验组和对照组。实验组于显微镜下在角巩缘做约 1.5mm宽隧道切口 ,使用 5号一次性注射针头 ,尖端弯成 90度角 ,伸入前房钩取、破坏角膜内皮层 ,镜下可见内皮层脱落 ,破坏面积约占角膜内皮面的 80 %。术后 1～ 4w后进行组织学观察。结果 :术后 10 0 %实验兔发生BK ,范围达 10 0 %。肉眼及显微镜下可见角膜明显水肿 ,呈灰白色混浊 ,表面呈大泡状 ;组织学切片显示BK眼角膜明显增厚 ,上皮细胞有水肿、退变、间隙积液、大泡形成、表面不规则、少量炎症细胞浸润 ,角膜基质排列紊乱 ,细胞间隙明显增宽 ,后期近角膜上皮侧有新生血管形成。结论 :通过破坏兔眼角膜内皮层的方法建立BK动物模型 ,方法简便、实用、有效 。
Objective:To develop an experimental animal model for bullous keratopathy(BK) using the rabbit cornea in order to lay the groundwork for further research on impairment mechanisms and methods of treatment.Methods:Eleven pigmented New Zealand rabbits were divided into three experimental groups and two control groups. With the aid of a microscope,a 1.5 millimeter wide tunnel incision was made at the corneal margin in all experimental groups. A No.5 syringe needle was injected at a ninety degree angle to the tip and at an obtuse angle 10 millimeters from that point a 5 ml syringe was used to inject a balanced salt solution. The needle entered into the anterior chamber through the tunnel. The syringe was used to inject the solution,to maintain the balance of pressure in the anterior chamber and to remove and destroy corneal epithelium as well. Epithelial cells were scaled off the cornea and the destroyed area accounted for about 70 to 80 per cent of the cornea,which was observed under the microscope. Chloromycetin eye drops were applied 4 times a day to prevent infection. After an observation period of 1 to 4 weeks,rabbits were sacrificed by injecting an excessive amount of 3% pentobarbital sodium in the aurismarginal vein. A digital camera was used to take pictures and the condition of the cornea was examined under the microscope. The cornea was then removed after the eyeball was extracted. The cornea was preserved with 10% formalin,dehydrated by alcohol,infused with wax and disposed by HE section staining. The section was then observed and photographed under an optical microscope(Olympus Bx4,made in Japan).Results:There was some yellow white secretion in 6 conjunctival sacs,moderate congestion in all conjuctivae,and obvious hydrops and hoar turbidity in all corneas on the first day after surgery. Two days later,the bullous surfaces of all corneas had changed,and the conditions of the anterior chamber and the back section of the eye were difficult to observe. From 2.5 to 3 weeks,neovascularization gradually increased from the margin to the center between the layers of the cornea. At the end of the 2nd week,histology slices indicated that the BK cornea had noticeably increased to 4～4.5 times of normal corneal thickness. With epithelium hydrops,intermittent dropsy,bullation,surface abnormality,infiltration by a small quantity of lymphocytes,and an abnormal arrangement of the stromal layer of the cornea,with widened intervals between stromal cells,were observed. At the end of the 4th week,the epithelia of the cornea were abnormally arranged and infiltrated by lymphocytes with hyperplasia and the growth of neovascular vessels underneath.Conclusion:The establishment of an experimental animal model on BK by destroying the endothelial layer of the rabbit's cornea is a convenient,practical and effective method for use in research. It can lay the groundwork for further research on the pathological changes and methods of treatment of BK.
出处
《眼视光学杂志》
2002年第4期225-227,共3页
Chinese Journal of Optometry & Ophthalmology
基金
广东深圳市 2 0 0 1年度科技基金资助项目 ( 2 0 0 10 414 9)
