摘要
目的 探讨地塞米松 (DXM )对大鼠肾小球系膜细胞 (GMCs)增生作用的影响。方法 培养大鼠GMCs细胞 ,分空白对照组、LPS组和DXM组。DXM选用 3种浓度 ,分别为 2 5 0ng/孔、10 0 0ng/孔和 4 0 0 0ng/孔。LPS终浓度为 5mg/孔 。采用MTT掺入方法 ,于 2 4h和 4 8h观察 3组中GMCs增生水平。结果 DXM组在浓度为 4 0 0 0ng/孔时 4 8h的增生水平为 (0 .2 5 1± 0 .0 5 6 )ng/L ,低于LPS组和GMCs组 [(0 .787± 0 .110 ) ,(0 .6 78± 0 .10 1)ng/L],差异有显著性 (P <0 .0 5 )。DXM浓度为 4 0 0 0ng/孔时GMCs增生水平于 4 8h抑制最为显著。结论 DXM能抑制大鼠GMCs增生。
Objective To study the effect of dexamethasone (DXM) on the proliferation of rat glomerulus mesangial cells (GMCs). Methods The GMCs lines of the rat were cultured in virto. Both DXM and lipopolysaccharide (LPS) or LPS alone were added into the culture medium (DXM group or LPS group); in the others, neither DXM nor LPS was added and they were used as controls. The GMCs proliferative level was detected by the MTT method at 24 and 48 h of the experiment. Results The GMCs proliferative level in the DXM group of 4 000 ng/well at 48 h [( 0.251 ± 0.056 ) ng/L] was lower than that of the LPS group [( 0.787 ± 0.110 ) ng/L] and the controls [( 0.678 ± 0.101 ) ng/L](P< 0.05 ); and the GMCs proliferative inhibition reached a peak at 48 h. Conclusions DXM may inhibit the proliferation of GMCs in rats.
出处
《中国当代儿科杂志》
CAS
CSCD
2002年第6期443-444,447,共3页
Chinese Journal of Contemporary Pediatrics
基金
国家自然科学基金资助课题 (No .3 980 0 14 0 )
湖南省卫生厅科研立项课题 (No .2 0 0 1-Y88)
