摘要
目的 研究LMP1基因C端区缺失突变在我国南方广东、广西鼻咽癌高发区的存在状况和探讨其在鼻咽癌中所起的作用。方法 采用聚合酶链反应技术 (PCR)扩增鼻咽癌患者鼻咽组织中LMP1基因的C末端 ,并对其进行了克隆和序列分析。结果 2 0份鼻咽癌组织标本中 ,有 17份扩增出特异性条带 ,阳性率为 85 % ,阳性个体中只有 1份未发生缺失。我们选择其中的 4份样品进行了克隆和序列分析 ,结果显示 ,4份样品均存在 3 0个碱基的缺失和某些位点的单点突变。结论 广东。
Objective To study the deletion and mutation in carboxy terminal region of LMP1 gene derived from nasopharyngeal carcinoma (NPC) in Guangdong and Guangxi, the high risk areas of nasopharyngeal carcinoma in China. Methods LMP1 gene carboxy terminal region was amplified from nasopharyngeal carcinoma tissues by PCR, and then cloned and sequenced. Results Of the 20 cases, 17 were LMP1 positive. In all positive cases, only 1 case did not show deletion. Four positive cases were chosen for DNA sequencing, The rusult showed that all the four cases had mutation and the 30bp deletion. Conclusion High frequency of deletion and mutation in LMP1 gene of nasopharyngeal carcinoma tissues was found in Guangdong and Guangxi. Whether it related to the high incidence of NPC should be further studied.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2003年第1期35-38,共4页
Chinese Journal of Experimental and Clinical Virology
基金
国家自然科学基金重点项目 (39830 380 )
关键词
鼻咽癌
LMP1基因
突变
序列分析
Nasopharyngeal Neoplasms
Genes,LMP1
Mutation
Sequence Analysis
