摘要
基于全基因组重测序进行分子标记开发是一种新的方法和趋势。本研究基于辣椒全基因组重测序数据鉴定出的InDel位点,在2号染色体上随机选择并设计了40个InDel标记,以24份辣椒种质验证其有效性。结果显示:40个标记可获得扩增产物,其中具有多态性的标记35个,多态率为87.5%,同时35个标记可以有效地区分24份辣椒种质的多样性和特异性。以上结果表明,基于重测序数据开发辣椒InDel标记切实可行。
The development of molecular markers based on genome re?sequencing is a new method and trend. In this research, forty InDel loci on chromosome 2 of pepper were chosen randomly and designed by screening the InDel loci that had been identified based on pepper genome re?sequencing data. The effectiveness of InDel markers were tested on 24 pep?per germplasm. Among the forty InDels, thirty?five were polymorphisc, accounting for 87.5% of the total, and could reveal the genetic diversity and specificity of the tested pepper germplasm, indicating that development of pepper InDel markers based on genome re?sequencing data was practicable.
出处
《江苏农业学报》
CSCD
北大核心
2015年第6期1400-1406,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31401866)
江苏省自然科学基金项目(4111415)
江苏省农业科技自主创新基金项目[CX(14)5013]
国家高技术研究发展计划(863计划)项目(2012AA100103)
现代农业产业技术体系建设专项基金项目(CARS-25)
关键词
辣椒
重测序
INDEL标记
种质鉴定
pepper
re-sequencing
InDel marker
germplasm identification
