摘要
目的了解2014—2018年浙江省丽水市副溶血弧菌(Vp)的流行情况、血清型别、携带毒力基因情况及分子分型特征,为预防食源性疾病提供依据。方法分别收集2014—2018年丽水市食源性疾病监测中的11 420例临床腹泻患者粪便与食品安全风险监测中的497份食品标本,进行Vp的分离培养,分析阳性菌株的分布特征。同时对菌株开展血清分型,毒力基因检测和脉冲场凝胶电泳(PFGE)分子分型。结果共分离到296株Vp,其中251株来源于腹泻患者粪便,45株来源于食品。丽水市Vp流行集中于第三季度,患者集中于成年人,且在家庭用餐占多数。患者来源菌株主要血清型为O3∶K6,毒力基因型为tdh+trhtlh+,食品来源菌株主要血清型为O2,毒力基因型为tdh-trh-tlh+。48株Vp可分为42个PFGE带型,带型相似值为6.16%~100.00%。结论第三季度为Vp的卫生监管重点,要加强对饮食习惯尤其是家庭用餐时对水产品正确处理方面的宣教。丽水市目前流行的菌株基因相似度低,可能存在多克隆来源。重点监测血清型患者为O3∶K6,食品为O2。
Objective To investigate the spread,serotype,virulence gene and molecular typing characteristics of Vibrio parahaemolyticus in Lishui of Zhejiang province and provide evidence for the prevention of foodborne diseases.Methods A total of 11420 stool samples of clinical diarrhea patients in foodborne disease surveillance and 497 food samples in food safety risk surveillance were collected in Lishui from 2014 to 2018 for the isolation and culture of V.parahaemolyticus,and the distribution characteristics of positive strains were analyzed.Serological typing,virulence gene detection and pulsed field gel electrophoresis(PFGE)for molecular typing were performed for the strains.Results A total of 296 strains of V.parahaemolyticus were isolated,in which 251 were from diarrhea patients and 45 were from foods.V.parahaemolyticus was mainly detected during July-September,The cases were mainly adults and most of them usually dined at home.O3∶K6 was the main serotype and tdh+trh-tlh+was the virulence genotype detected in patients.O2 was the main serotype and tdh-trhtlh+was the virulence genotype detected in foods.Forty eight strains of V.parahaemolyticus could be classified into 42 PFGE banding patterns,and the banding pattern similarity values were from 6.16%to 100.00%.Conclusion It is necessary to strengthen the health supervision for V.parahaemolyticus infection and health education about having a good dining habit and improving the household cooking process of aquatic products during July-September.The genetic similarity of the V.parahaemolyticus strains spreading in Lishui was low and polyclonal sources might exist.Serotype O3∶K6 was mainly detected in patients,and O2 was mainly in foods.
作者
陈秀英
梅建华
陈沙彬
柳付明
叶夏良
Chen Xiuying;Mei Jianhua;Chen Shabin;Liu Fuming;Ye Xialiang(Lishui Prefectural Center for Disease Control and Prevention,Lishui 323000,Zhejiang,China)
出处
《疾病监测》
CAS
2019年第9期822-826,共5页
Disease Surveillance
基金
浙江省公共卫生应急检测关键技术重点实验室开放基金(浙疾发[2018]33号)~~
关键词
副溶血弧菌
血清型
毒力基因
分子分型
脉冲场凝胶电泳
Vibrio parahaemolyticus
Serotype
Virulence gene
Molecular typing
Pulsed field gel electrophoresis
作者简介
陈秀英,女,浙江省丽水市人,硕士,副主任技师,主要从事细菌学研究,Email:68327655@qq.com;通信作者:叶夏良,Tel:0578-2057567,Email:594592794@qq.com
