摘要
根据已经发表的多酚氧化酶基因中的保守序列 ,设计兼并引物 ,利用nest-PCR技术 ,首次克隆了茶树多酚氧化酶基因。该基因已经在GenBank上登录 ,登录号为AF2 69192。序列分析表明 ,茶树多酚氧化酶基因与其它植物中的多酚氧化酶基因有较高的相似性 ,尤其在铜离子结合部位 ,所有的植物基本上一致。进化树分析表明 。
For the first time the polyphenol oxidase (PPO) gene in tea plant (Camellia sinensis (L.) O. Kuntze) was cloned, during which, the consensus sequences of previous published PPO were used to design the degenerate primers and reverse transcription by using nest_PCR method. The obtained PPO gene of tea plant was registered in GenBank, whose access number is AF269192. Alignment of sequences with ClustalW indicates that the PPO gene of tea plant has high homology with those found in other plants, especially copper binding regions. The polyphenol oxidase of the tea plant can be clustered in the same group with most other woody plants according to phylogenetic tree analysis.
出处
《茶叶科学》
CAS
CSCD
北大核心
2001年第2期94-98,共5页
Journal of Tea Science
基金
国家自然科学基金资助项目 (39770 4 75)
关键词
茶树
多酚氧化酶基因
克隆
Tea plant (Camellia sinensis (L.) O. Kuntze)
Polyphenol oxidase (PPO)
Gene
Cloning
