摘要
落叶松种间杂交的结实率普遍较高 ,但不同种之间有一定差异 ,其中日本落叶松与兴安落叶松交配结实率最低。种内自由授粉和自交的对比试验显示 ,空粒种子产生的主要原因是自交。本文讨论了自交不孕无性系的可能利用途径。在不同交配组合下研究了华北落叶松捕捉花粉的过程 ,确定在传粉期花粉和雌性生殖器官存在一定的相互作用 ,但无特异性。传粉后约 4周 ,珠心顶端破裂 ,分泌物进入珠孔道 ,并与珠被分泌物共同作用于花粉 ,使花粉向珠心移动。组织化学分析表明 ,从传粉到受精期间 ,蛋白质的分布发生区域性变化 ,传粉期蛋白质几乎分布于整个胚珠 ,而在受精期则主要集中在颈卵器之上的珠心组织中。初步确定在花粉萌发和受精期 ,华北落叶松中存在花粉识别与选择系统。
Generally the fruiting ability of interspecific crossing in Larix is high, although there are differences between different crosses. The fruiting ability of the cross between L. kaempferi and L. gmelini was the lowest. It was revealed that the emptied seeds were mainly due to the self pollination through the experiments of open and self pollination. Some clones were almost total self incompatibility. The possible usage of these clones in seed orchard was discussed. Compared the process of pollen capture with different pollen (open , self pollens of L. principis rupprechetii and pollen of Pinus tabulaeformis ) under control pollination, there was no selection between ovule and pollen at the pollination stage, and all kinds of pollen could enter into ovule canal. About 4 weeks after pollination, the tip cells of nucellus broke and secreted liquid into ovular canal, which promoted pollen movement to the nucellus and played important role for pollen germination. Through the histo chemical analysis, the distribution of protein changed from pollination to fertilization stage. At pollination stage, protein almost distributed in whole ovule, while at fertilization stage it gradually transferred to the upper tissue of nucellus. The specialties between ovule and pollen during pollen germination and fertilization were detected.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2003年第1期62-68,共7页
Scientia Silvae Sinicae
基金
国家自然科学基金资助项目 (3 9970 62 3 )
