摘要
目的探讨石见穿多糖诱导人肝癌HepG2细胞凋亡作用。方法荧光显微镜观察细胞凋亡的形态学改变;利用流式细胞仪Annexin V-FITC/PI法检测经不同浓度石见穿多糖作用的肝癌HepG2细胞的凋亡率。结果荧光显微镜观察可见HepG细胞不同时期凋亡形态学改变;石见穿多糖各浓度组平均细胞凋亡率分别为25mg/L组(8.27±1.84)%、50mg/L组(13.53±2.67)%、100 mg/L组(22.03±2.48)%,显著高于对照组(0.57±0.23)%(P<0.01)。结论石见穿多糖能够诱导肝癌细胞HepG2凋亡,且凋亡率随着石见穿多糖浓度的增加而增高。
Objective To investigate the polysaccharides induced apoptosis in human hepatoma HepG2 cells apoptosis. Methods Use fluorescence microscopy to observe cell apoptotic morphological changes; use flow cytometry of Annexin V-FITC / PI to detect different concentration of polysaccharides of hepatoma HepG2 cells apoptosis. Results Fluorescence can observe the changes of microscope HepG cell apoptotic morphological in different periods average cell apoptosis rate of polysaccharides with different concentration groups were 25mg / L(8. 27 ± 1. 84) %,50mg / L(13. 53 ± 2. 67) %,100 mg / L group(22. 03 ± 2. 48) %,they were significantly higher than that of control group(0. 57 ± 0. 23)%(P <0. 01). Conclusion Polysaccharides can induce the apoptosis of hepatoma cell line HepG2,and increase the rate of apoptosis with polysaccharides concentration.
出处
《湖北中医药大学学报》
2014年第2期17-18,共2页
Journal of Hubei University of Chinese Medicine
基金
湖北省自然科学基金项目(DB328)
关键词
石见穿多糖
肝癌
凋亡
流式细胞术
Salvia chinensis Benth polysaccharides
hepatocellular carcinoma
apoptosis
flow cytometry
