摘要
希金斯炭疽菌可以侵染诸多十字花科植物引起炭疽病,给各国农业生产造成了巨大经济损失.GPCR作为生物体内G蛋白信号转导途径中的重要感知蛋白,在信号传递过程中发挥着重要作用.本研究基于酿酒酵母中已经报道的3个典型GPCR序列,利用Blastp以及关键词对炭疽菌蛋白质数据库进行比对、搜索,以及通过TMHMM、HMMTOP跨膜结构域分析,明确该菌存在4个典型的GPCR;同时,通过对上述氨基酸序列进行细胞信号肽、亚细胞定位以及二级结构等生物信息学分析,明确上述GPCR均具有较高比例的α螺旋结构以及均不含有明显的信号肽序列;在定位方面,4个GPCR均定位在质膜上.此外,通过对希金斯炭疽菌中的4个GPCR与其他物种中的23个同源序列进行遗传关系比较分析,发现该菌中的GPCR与C.graminicola、C.fioriniae等炭疽菌属中的病菌具有较高的同源序列以及较近的亲缘关系.该研究为深入开展希金斯炭疽菌GPCR功能研究打下坚实的理论基础,同时,也为进一步开展其他炭疽菌的研究提供重要的理论指导.
Colletotrichum higginsanum can infect cruciferous vegetables plants,causing tremendous economic losses in agricultural production in many countries.GPCR as a perception protein of G protein signal transduction in vivo plays an important role in the signal transduction process.Based on the three typical GPCR sequences had been reported in Saccharomyces cerevisiae,to search GPCR-like protein sequence from the protein databases of Colletotrichum spp.with the Blastp as well as the use of keywords,and the seven trans-membrane domain of four typical GPCRs were analyzed by using the TMHMM.Meanwhile,through bioinformatics analysis including the signal peptide,subcellular location and the secondary structure,there is high proportion of helical structure and not contains significant signal peptide sequence in every GPCR protein,and which positioned in the plasma membrane.In addition,analysis of genetic relationships through comparative four GPCRs in C.graminicola with 23 homologous sequences in other species,both C.graminicolaand C.fioriniae have a high sequence homology and close genetic relationship with C.higginsanum.This study could provide strong theoretical foundation to the function of GPCR,and provided an important theoretical guidance to clarify the other pathogen in Colletotrichum spp.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2015年第2期246-251,共6页
Journal of Central China Normal University：Natural Sciences
基金
云南省优势特色重点学科生物学一级学科建设项目(50097505)
云南省高校林下生物资源保护及利用科技创新团队(2014015)
云南省教育厅科学研究基金项目(2014Y330)
国家自然科学基金青年科学基金项目(31200488)
