摘要
本文以一水柠檬酸和尿素为原料,通过微波法合成氮掺杂碳点(N-CDs)。利用透射电子显微镜(TEM)、X-射线衍射法(XRD)、X射线光电子能谱(XPS)、傅里叶变换红外光谱(FT-IR)、紫外可见吸收光谱(UV-Vis)和荧光光谱对合成的N-CDs进行表征。N-CDs的荧光强度随着蛇床子素浓度的增加而逐渐被猝灭。在最佳实验条件下,N-CDs荧光探针对蛇床子素有较高的选择性和灵敏性。蛇床子素在5×10^(-6 )M～7.5×10^(-5 )M的浓度范围内与N-CDs的荧光强度呈良好的线性范围,检出限为3.8×10^(-9) M。基于此建立了一种以氮掺杂碳点(N-CDs)为荧光探针快速测定蛇床子素的方法。同时讨论了蛇床子素与N-CDs相互作用的机理,此方法已被应用于实际血样和尿样中微量蛇床子素的测定。
Nitrogen doped carbon dots(N-CDs)were synthesized by microwave synthesis method using citric acid monohydrate and urea as raw materials.The N-CDs were fully characterized by transmission electron microscopy(TEM),x-ray diffractometry(XRD),x-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FT-IR),ultraviolet-visible spectroscopy(UV-Vis)and fluorescence spectroscopy.The fluorescence intensity of N-CDs were quenched with the increasing of osthole concentration.Under the optimal experimental conditions,the N-CDs fluorescence probe is highly selective and sensitive to Osthole.The linear response range for Osthole is 5×10-6 M^7.5×10-5 M with a detection limit of 3.8×10-9 M.A rapid method has been developed for the determination of Osthole using the Nitrogen doped carbon dots(N-CDs)as a fluorescent probe.Moreover,the mechanism of the interaction of Osthole and N-CDs is discussed.The method has been applied to the analysis of blood samples and urine samples.
作者
李志霞
杨学芳
孟鑫
吕俊杰
卞伟
LI Zhi-xia;YANG Xue-fang;MENG Xin;LV Jun-jie;BIAN Wei(School of Basic Medical Science,Shanxi Medical University,Taiyuan 030001,China;College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China)
出处
《化学研究与应用》
CAS
CSCD
北大核心
2019年第3期493-502,共10页
Chemical Research and Application
基金
山西省自然科学基金项目(201601D011018)资助
山西医科大学博士启动基金项目(03201514)资助
山西医科大学创新基金项目(01201312)资助
关键词
氮掺杂碳点
荧光探针
蛇床子素
Nitrogen doped carbon dotsl
Fluorescent probe
Osthole
作者简介
通讯作者:卞伟(1972-),女,教授,主要从事分子光谱分析研究。Email:sxykdx_bianwei@163.com。
