摘要
将小鹅瘟病毒 (GPV)通过家禽胚胎传代增殖 ,取感染胚尿囊液制备了GPV沉淀抗原。其抗原在琼脂扩散反应和对流免疫电泳的试验中能够与小鹅瘟阳性血清出现沉淀线 ,而且与小鹅瘟标准抗原的沉淀线相吻合。所形成的沉淀反应能够被小鹅瘟阳性血清特异性地抑制 ,与其它常见的禽病血清不发生交叉反应。
The gosling plague viruses (GPV) were propagated and passaged on avian embryos.The GPV precipitation antigen was prepared with chorioallantoic fluid collected from infected embryos.This antigen can react with positive serum of gosling plague,form precipitation lines in agar gel precipitation and counter immuno electrophoresis.The precipitation lines are coincided with standard antigen of gosling plague.Furtheremore this precipitation reaction can be specifically inhibited by positive serum of gosling plague and no cross reaction is observed with common sera of avian disease.The results show that this GPV antigen can be used for surveillance of immunological effects and investigation of veterinary epidemiology.
