摘要
【目的】草地贪夜蛾是原产于美洲热带和亚热带地区的重大迁飞性农业害虫。海南省是其北迁虫源地之一,明确海南省草地贪夜蛾生物型和遗传特征,对全省及全国草地贪夜蛾的监测与防控具有重要意义。【方法】利用线粒体基因COⅠ和核基因Tpi 2种分子标记,对采自海南省玉米植株上的76个草地贪夜蛾样本进行分子鉴定及遗传特征分析。【结果】线粒体基因COⅠ的分析结果显示,海南省草地贪夜蛾样本中76.3%为水稻型,23.7%为玉米型;核基因Tpi的分析结果表明,海南草地贪夜蛾大部分为玉米型,且可能存在水稻与玉米杂合型。基于COⅠ基因建立的进化树显示,海南省的草地贪夜蛾与我国广东、美国佛罗里达和南非的COⅠ序列一致。【结论】本研究明确了海南省草地贪夜蛾生物型及分布,为海南省早期监测预警及精准防控提供了参考依据。
【Aim】Spodoptera frugiperda is a major migratory agricultural pest that is native to the tropical and subtropical regions of the Americas.Hainan is one of the source areas of this pest for migration in China.Revealing the biotypes and genetic characteristics of S.frugiperda in Hainan will be beneficial for monitoring and prevention of this pest in Hainan and even China.【Method】Two molecular markers,the mitochondrial gene COⅠand the nuclear gene Tpi,were used for identification and genetic characterization of 76 samples from corn plants in Hainan.【Result】According to COⅠsequences,76.3%of samples were the rice strain and 23.7%were the corn strain.According to Tpi sequences,most samples were the corn strain,and there might be hybrids of rice and corn strains.The phylogenetic tree built based on COⅠindicated that S.frugiperda in Hainan has the same COⅠsequence as those in Guangdong(China),Florida(USA),and South Africa.【Conclusion】This study clarifies the biotypes and distribution of S.frugiperda in Hainan,providing a reference for early monitoring,warning,and precise control of this pest in Hainan.
作者
马光昌
唐超
龚治
金涛
温海波
MA Guangchang;TANG Chao;GONG Zhi;JIN Tao;WEN Haibo(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China)
出处
《生物安全学报(中英文)》
北大核心
2025年第4期324-330,376,共8页
Journal of biosafety
基金
海南省自然科学基金面上项目(320MS082)。
关键词
草地贪夜蛾
生物型
分子标记
遗传特征
Spodoptera frugiperda
biotype
molecular marker
genetic characteristics
作者简介
马光昌,男,副研究员。研究方向:入侵生物监测及生物防治。E-mail:mgc53@126.com;通信作者:温海波,E-mail:wenhaibo@163.com。
