摘要
[目的]探讨雨生红球藻粉(Haematococcus pluvialis,H. pluvialis)对脂多糖(LPS)诱导的口腔上皮损伤的保护作用。[方法]检测不同剂量的H. pluvialis对人正常口腔上皮角质细胞(HOKs)活力的影响;测定LPS诱导的HOKs中紧密连接相关蛋白ZO-1和Occludin、线粒体形态、活性氧(ROS)、线粒体膜电位(MMP)、线粒体超氧化物(MitoSOX)和线粒体通透性转换孔(MPTP)的变化。[结果]与LPS模型组相比,高剂量H. pluvialis显著上调Occludin和ZO-1蛋白表达水平分别至151.56%和170.52%(P<0.05),并且促进了Occludin和ZO-1蛋白的细胞膜分布。此外,H. pluvialis明显改善了LPS诱导的HOKs中产生过量的ROS、MitoSOX、MMP的降低和MPTP增大。[结论]H. pluvialis可能通过改善线粒体功能障碍和降低氧化应激水平改善LPS诱导的HOKs屏障损伤,提示H. pluvialis可开发为口腔黏膜屏障修复的功能性食品。
[Objective]To investigate the protective effect of Haematococcus pluvialis(H.pluvialis)powder on lipopolysaccharide(LPS)-induced oral epithelial damage.[Methods]The effects of different doses of H.pluvialis on the viability of human oral keratinocytes(HOKs)were assessed.In LPS-induced HOKs,changes in the tight junction-associated proteins ZO-1 and Occludin,mitochondrial morphology,reactive oxygen species(ROS),mitochondrial membrane potential(MMP),mitochondrial superoxide(MitoSOX),and mitochondrial permeability transition pore(MPTP)were evaluated.[Results]Compared with the LPS model group,high-dose H.pluvialis significantly upregulated the expression levels of Occludin and ZO-1 to 151.56%and 170.52%,respectively(P<0.05),and promoted their membrane localization.In addition,H.pluvialis markedly improved LPS-induced excessive ROS and MitoSOX production,the reduction in MMP,and the increase in MPTP in HOKs.[Conclusion]H.pluvialis may alleviate LPS-induced barrier damage in HOKs by improving mitochondrial dysfunction and reducing oxidative stress,suggesting its potential as a functional food for oral mucosal barrier repair.
作者
谢金梅
陈彩炼
张勇
郑云剑
谢曦
XIE Jinmei;CHEN Cailian;ZHANG Yong;ZHENG Yunjian;XIE Xi(School of Food Science and Engineering,Hainan University,Haikou,Hainan 570228,China;Key Laboratory of Tropical Biological Resources of Ministry of Education,Hainan University,Haikou,Hainan 570228,China;School of Pharmaceutical Sciences,Hainan University,Haikou,Hainan 570228,China;Aierfa Biotechnology Co.,Ltd.,Haining,Zhejiang 314400,China)
出处
《食品与机械》
北大核心
2025年第9期137-146,共10页
Food and Machinery
基金
海南省自然科学基金项目(编号:823RC467,821MS027,822MS054)
国家自然科学基金项目(编号:82360731)
科技人才与平台计划(院士专家工作站,云南省孙树侠专家工作站,编号:202305AF150114)。
关键词
雨生红球藻粉
功能性食品
上皮细胞屏障功能
线粒体功能障碍
氧化应激
Haematococcus pluvialis
functional food
epithelial cell barrier function
mitochondrial dysfunction
oxidative stress
作者简介
通信作者:谢曦(1986-),女,海南大学教授,博士。E-mail:xiexi@hainanu.edu.cn。
