摘要
研究旨在比较妊娠新西兰兔应激性腹泻对胚胎-胎仔发育(EFD)试验各指标的影响。挑取广东莱恩医药研究院有限公司2020—2023年EFD试验中阴性对照组妊娠兔,统计分析腹泻对母体妊娠期体重、妊娠情况以及胎仔生长发育等方面指标的影响。结果显示,与无腹泻组(n=73)相比,腹泻组(n=17)新西兰兔母体妊娠期第10~第29天的体重以及总增重、子宫连胎重、子宫重极显著降低(P<0.01)。与无腹泻组相比,腹泻组胚胎着床后死亡率、吸收胎率极显著升高(P<0.01),平均活胎数显著降低(P<0.05),着床率、活胎率极显著降低(P<0.01);去除窝效应后,着床后死亡率、吸收胎率仍显著升高(P<0.05),活胎率显著降低(P<0.05)。无腹泻组产胎仔710只,腹泻组产胎仔119只。与无腹泻组相比,腹泻组胎仔体重、胎盘重量极显著降低(P<0.01),顶臀长、尾长极显著缩短(P<0.01)。无腹泻组有1例胎仔无尾,异常率为0.14%。进行头部检查的胎仔共388例,包括无腹泻组332例,腹泻组56例。腹泻组胎仔内脏总畸形率(去除窝效应)为0.79%,略高于无腹泻组;腹泻组骨骼总畸形率(去除窝效应)为2.50%,略低于无腹泻组。两组胎仔第二脑室扩张、腹腔积液以及胸骨点5个、胸骨点0~4个、胸骨畸形、胸骨骨化不全、肋骨畸形、前后肢带骨畸形等异常率差异均不显著(P>0.05)。研究表明,妊娠新西兰兔应激性腹泻主要增加吸收胎率,对EFD研究的关键性评价指标(外观、内脏、骨骼)无显著影响。
The study aims to compare the effects of stress-induced diarrhea in pregnant New Zealand rabbits on various indicators of the embryo-fetal development(EFD)test.Pregnant rabbits in the negative control group from the EFD trials of Guangdong Lewwin Pharmaceutical Research Institute Co.,Ltd.from 2020 to 2023 were selected.The effects of diarrhea on the maternal weight,pregnancy status,and the growth and development of the fetus during pregnancy were statistically analyzed.The results showed that compared with the non-diarrhea group(n=73),the body weight,total weight gain,combined fetal weight and uterine weight of New Zealand rabbits in the diarrhea group(n=17)from the 10th to the 29th day of gestation were extremely reduced(P<0.01).Compared with the non-diarrhea group,the mortality rate and absorption rate of embryos after implantation in the diarrhea group were extremely increased(P<0.01),the average number of live births was significantly decreased(P<0.05),and the implantation rate and live birth rate were extremely decreased(P<0.01).After removing the pit effect,the mortality rate and absorption rate after implantation still increased significantly(P<0.05),while the live birth rate decreased significantly(P<0.05).In the non-diarrhea group,710 fetuses were delivered,while in the diarrhea group,119 fetuses were delivered.Compared with the non-diarrhea group,the fetal weight and placental weight in the diarrhea group were extremely reduced(P<0.01),and the apical hip length and tail length were extremely shortened(P<0.01).In the non-diarrhea group,there was one case of capless fetus,with an abnormal rate of 0.14%.A total of 388 fetuses underwent head examinations,including 332 cases in the non-diarrhea group and 56 cases in the diarrhea group.The total malformation rate of fetal internal organs in the diarrhea group(excluding the pit effect)was 0.79%,slightly higher than that in the non-diarrhea group.The total bone deformity rate(excluding pit effect)in the diarrhea group was 2.50%,slightly lower than that in the non-diarrhea group.There were no significant differences in the abnormal rates of second ventricle dilation,peritoneal effusion,five sternal points,0 to 4 sternal points,sternal deformity,sternal ossification defect,rib deformity,and anterior and posterior limb band deformity between the two groups(P>0.05).Studies have shown that stress-induced diarrhea in pregnant New Zealand rabbits mainly increases the rate of absorbed fetuses and has no significant impact on the key evaluation indicators of EFD studies(appearance,internal organs,and bones).
作者
张发福
张文强
柳璐
牛慧君
杨威
郭健敏
Zhang Fafu;Zhang Wenqiang;Liu Lu;Niu Huijun;Yang Wei;Guo Jianmin(Guangzhou Bay Area Institute of Biomedicine,Guangdong Lewwin Pharmaceutical Research Institute Co.,Ltd.,Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research,TCM Non-Clinic Evaluation Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine,Guangdong Engineering Research Center for Innovative Drug Evaluation and Research,Guangdong Guangzhou 510990)
出处
《现代畜牧兽医》
2025年第9期7-12,共6页
Modern Journal of Animal Husbandry and Veterinary Medicine
基金
国家科技创业领军人才(SQ2024RA3E000198)
广东省重大人才工程项目(2021TY060021)
广东省药物非临床评价与研究重点实验室(2024)。
关键词
妊娠新西兰兔
应激性腹泻
胚胎-胎仔
发育毒性
Pregnant New Zealand rabbit
Stress-induced diarrhea
Embryo-fetal
Developmental toxicity
作者简介
张发福,硕士,研究方向为新药药理毒理。;通讯作者:郭健敏,博士,正高级工程师,研究方向为新药药理毒理。
