摘要
目的探讨持续被动运动(CPM)联合富血小板血浆(PRP)治疗对兔膝骨关节炎(KOA)模型的软骨保护作用及Wnt/β-catenin信号通路的影响。方法建立新西兰大白兔KOA模型,采用随机数字表法将KOA模型分为KOA组、CPM组、PRP组、CPM+PRP组;另选取假手术的新西兰大白兔作为Sham组。CPM组兔在CPM训练仪上进行膝关节CPM,1次/d,每次运动40 min;PRP组在兔关节腔内注射PRP,1次/d,每次注射0.3 mL;CPM+PRP组兔进行CPM 40 min后在关节腔内注射PRP 0.3 mL,1次/d;Sham组、KOA组正常饲养不予以治疗。共干预8周。干预结束后,采用硝酸还原酶法检测兔膝关节滑膜液中一氧化氮(NO)水平;采用酶联免疫吸附试验检测兔膝关节滑膜液中白细胞介素(IL)-1β及软骨组织中基质金属蛋白酶(MMP)-1、MMP-13水平;采用实时荧光定量反转录聚合酶链反应、蛋白质免疫印迹法分别检测兔膝关节软骨组织中Wnt2、β-catenin mRNA及Wnt2、β-catenin蛋白水平。结果KOA组兔膝关节滑膜液中NO、IL-1β水平及软骨组织中MMP-1、MMP-13水平、Mankin's评分均高于Sham组,软骨组织中Wnt2、β-catenin mRNA及Wnt2、β-catenin蛋白水平均明显低于Sham组,差异均有统计学意义(P<0.05)。CPM组、PRP组及CPM+PRP组兔膝关节滑膜液中NO、IL-1β水平及软骨组织中MMP-1、MMP-13水平、Mankin's评分均明显低于KOA组,软骨组织中Wnt2、β-catenin mRNA及Wnt2、β-catenin蛋白水平均明显高于KOA组,且CPM+PRP组均优于CPM组、PRP组,差异均有统计学意义(P<0.05)。结论CPM联合PRP治疗可更好地减轻KOA炎症反应及软骨组织病变,保护软骨组织免受降解,可能与促进Wnt/β-catenin信号通路激活有关。
Objective To investigate the protective effect of continuous passive motion(CPM)combined with platelet-rich plasma(PRP)on cartilage and Wnt/β-catenin signaling pathway in rabbit knee osteoarthritis(KOA)model.Methods The KOA model of New Zealand white rabbits was established and randomly divided into KOA group,CPM group,PRP group and CPM+PRP group.Another New Zealand white rabbits with Sham operation were selected as sham group,and the rabbits in CPM group underwent CPM on the CPM training instrument once a day for 40 min each time.Rabbits in PRP group were injected with PRP into the joint cavity once a day,0.3 mL each time.Rabbits in CPM+PRP group were injected with 0.3 mL PRP into the joint cavity after 40 min of CPM,once a day.The Sham group and the KOA group were fed normally without treatment for 8 weeks.After the intervention,the level of nitric oxide(NO)in the synovial fluid of the knee joint was detected by nitrate reductase method.The levels of interleukin(IL)-1βin synovial fluid and matrix metalloproteinase(MMP)-1 and MMP-13 in cartilage tissue were detected by enzyme-linked immunosorbent assay.The mRNA and protein levels of Wnt2 andβ-catenin in rabbit knee cartilage were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction and Western blot respectively.Results The NO,IL-1βlevels and MMP-1,MMP-13 in synovial fluid and Mankin's score of cartilage tissue in KOA group were higher than those in Sham group,and the levels of Wnt2 andβ-catenin mRNA and Wnt2 andβ-catenin protein in cartilage tissue in KOA group were significantly lower than those in Sham group,and the differences were statistically significant(P<0.05).The NO,IL-1βlevels and MMP-1,MMP-13 in synovial fluid and Mankin's score of cartilage tissue in CPM group,PRP group and CPM+PRP group were significantly lower than those in KOA group,the levels of Wnt2,β-catenin mRNA and Wnt2,β-catenin protein in cartilage tissue were significantly higher than those in KOA group,and those in CPM+PRP group were significantly better than those in CPM group and PRP group(P<0.05).Conclusion CPM combined with PRP treatment can better reduce the inflammatory response and cartilage tissue lesions of KOA,and protect cartilage tissue from degradation,which may be related to promoting the activation of Wnt/β-catenin signaling pathway.
作者
黎小东
黄继超
杨理宁
田野
LI Xiaodong;HUANG Jichao;YANG Lining;TIAN Ye(Department of Sports Medicine Rehabilitation,Sports Hospital Affiliated to Chengdu University of Sport,Chengdu,Sichuan 610041,China)
出处
《检验医学与临床》
2025年第19期2700-2705,共6页
Laboratory Medicine and Clinic
基金
四川省中医药管理局科学技术研究专项课题(2020LC0066)。
作者简介
黎小东,男,中医师,主要从事中医骨伤、运动损伤及运动康复方面的研究。
