摘要
为系统评价玉米品种耐铝毒特性并建立高效筛选体系,对不同地区种植的45份杂交玉米品种采用土壤培养体系进行铝毒胁迫处理,运用隶属函数法、主成分分析、相关性分析、聚类分析和逐步回归分析等方法综合评估不同玉米种质资源耐铝毒特性并进行耐铝毒玉米品种筛选。在玉米3叶期这一关键生长阶段,测定茎粗、株高、SPAD值等12项形态生理指标,并引入铝毒耐性系数(C_(Alt))量化铝耐性水平。通过主成分分析降维提取特征指标,结合隶属函数法计算铝毒耐性综合评价值(D值),继而运用系统聚类将品种划分为5个耐性等级(其中,强耐铝毒型,D≥0.75;铝毒极敏感型,D≤0.25),筛选出强耐铝毒品种GY14022、EHD1、QKY900和HY1702,铝毒极敏感品种TR1818、JF86和ZY303等。结果显示,铝毒胁迫显著抑制玉米幼苗发育(P<0.001),株高和生物量降幅分别达5.37%和17.65%,各个耐性指标间存在显著相关性(P<0.05),验证了评价体系的可靠性。通过灰色关联度与逐步回归的双重验证,确立根系鲜质量、总干质量、SPAD值、总鲜质量和根长5个核心筛选指标。综上,铝毒害胁迫严重抑制了玉米苗期的生长,不同玉米品种耐酸性差异显著,筛选出根系鲜质量、总干质量、SPAD、总鲜质量和根长为苗期玉米耐铝毒筛选的关键指标。
To systematically evaluate the Al-toxicity of maize varieties and to establish an efficient screening system,45 hybrid maize varieties were selected to conduct Al-toxicity stress trials in this study.The experiment was designed in a dual-treatment mode:basic acidic soil(pH 5.15)and high-aluminumtoxic acidic soil to reveal the resistance mechanism of the varieties by comparing the differences in stress.During the critical growth stage of maize at the 3-leaf stage,the research team systematically measured 12 morpho-physiological indexes,including stem diameter,plant height,and SPAD value,and quantified the level of resistance by introducing the aluminum toxicity tolerance coefficient(C_(Alt)).In this study,we used a multidimensional analysis method to construct an evaluation system,firstly,we extracted the characteristic indexes by dimensional reduction of principal component analysis,combined with the affiliation function method to calculate the comprehensive resistance evaluation value(D value),and then we used the systematic clustering to classify the varieties into five resistance levels(among which extremely resistant D≥0.75,extremely sensitive D≤0.25),and then we screened out the extremely resistant varieties of aluminum toxicity,such as GY14022,EHD1,QKY900,and HY1702,and the aluminum toxicity resistant varieties of GY14022,EHD1,QKY900 and HY1702,and the aluminum toxicity resistant varieties of GY14022,EHD1,QKY900 and HY1702.HY1702,and extremely sensitive varieties TR1818,JF86 and ZY303.It was found that aluminum stress significantly inhibited the development of maize seedlings(P<0.001),the plant height and biomass decreased by 5.37%and 17.65%,respectively,and there was a significant correlation between the resistance indexes(P<0.05),which verified the reliability of the evaluation system.Finally,through the double validation of gray correlation and stepwise regression,the five core screening indexes of root fresh weight,total dry weight,SPAD value,total fresh weight and root length were confirmed.In conclusion,aluminum toxicity stress severely inhibited the growth of maize at seedling stage,and there were significant differences in acid tolerance among different maize varieties.The comprehensive analysis screened root fresh weight,total dry weight,SPAD,total fresh weight and root length as the key indexes for screening of aluminum toxicity resistance of maize at seedling stage.
作者
陈兴华
夏雨昕
李家鑫
丁广大
徐芳森
王创
CHEN Xinghua;XIA Yuxin;LI Jiaxin;DING Guangda;XU Fangsen;WANG Chuang(College of Resources and Environment,Microelement Research Center,Key Laboratory of Arable Land Conservation(Middle and Lower Reaches of Yangtze River),Ministry of Agriculture and Rural Affairs,Huazhong Agricultural University,Wuhan 430070,China)
出处
《华中农业大学学报》
北大核心
2025年第5期50-59,共10页
Journal of Huazhong Agricultural University
基金
国家重点研发计划项目(2022YFD1900705-4)。
关键词
杂交玉米
铝毒胁迫
主成分分析
隶属函数法
聚类分析
逐步回归分析
hybrid maize
aluminum toxicity stress
principal component analysis
affiliation function method
cluster analysis
stepwise regression analysis
作者简介
陈兴华,E-mail:cxh19970227@webmail.hzau.edu.cn;通信作者:王创,E-mail:chuang.wang@mail.hzau.edu.cn。
