摘要
【目的】为得出较为可靠的毛竹核心种质群体,促进毛竹种质有效利用和分子遗传学研究。【方法】本研究以432份毛竹种质为对象,基于15个表型特征,采用10%的总体取样率进行逐步系统聚类分析。结合2种遗传距离计算方法、3种取样技术和7种聚类算法,从多维度优化核心种质的取样方案。在确定最佳取样方案后,设置八种取样比例筛选最佳总体比例,并比较分组取样与未分组直接取样的效果。最终,通过多指标评估体系全面检验核心种质的代表性。【结果】采用10%取样比例、优先取样法、欧氏距离法和最短距离法相结合,是构建毛竹核心种质的最佳策略。不分组取样构建的最佳核心种质在方差差异百分率方面优于分组取样构建的核心种质,显示出更好的构建效果。通过表型性状的t检验、符合率检验和主成分分析,验证了所构建的43份核心种质能够有效避免遗传冗余,并充分代表原始种质。【结论】本研究首次构建了能够代表原始毛竹种质表型多样性的核心种质,为种质资源的收集、保护和有效利用提供支撑。
【Objective】This study aims to establish a reliable core germplasm population of Phyllostachys edulis(Moso bamboo),promoting its effective utilization and facilitating molecular genetics research.【Method】In this study,a total of 432 P.edulis germplasm accessions were targeted,and a stepwise systematic clustering analysis was conducted based on 15 phenotypic traits with an overall sampling rate of 10%.The sampling scheme for core germplasm was optimized from multiple dimensions,by incorporating two genetic distance calculation methods,three sampling techniques,and seven clustering algorithms.After determining the optimal sampling scheme,eight sampling proportions were set to screen the best overall proportion,and the effectiveness of group-based sampling was compared with direct sampling without grouping.Finally,the representativeness of the core germplasm was comprehensively evaluated through a multi-indicator assessment system.【Result】The study found that a combination of 10%sampling ratio,priority sampling method,Euclidean distance method,and minimum distance method was the optimal strategy for constructing the core germplasm of P.edulis.The core germplasm constructed by ungrouped sampling(NGcore)exhibited a higher variance difference percentage(VD)than that constructed with grouped sampling(LCore and PCore),indicating better performance.T-tests,coincidence rate tests,and principal component analysis of the phenotypic traits demonstrated that the selected 43 core accessions effectively avoided genetic redundancy and adequately represented the original germplasm.【Conclusion】This study has successfully constructed a core germplasm that represents the phenotypic diversity of the original P.edulis germplasm,laying a foundation for the collection,conservation,and effective utilization of germplasm resources.
作者
谷瑞
范少辉
魏松坡
刘广路
Gu Rui;Fan Shaohui;Wei Songpo;Liu Guanglu(International Center for Bamboo and Rattan,Beijing 100102;Key Laboratory of National Forestry and Grassland Administration for Bamboo&Rattan Science and Technology,Beijing 100102)
出处
《林业科学》
北大核心
2025年第9期101-112,共12页
Scientia Silvae Sinicae
基金
“十四五”国家重点研发计划项目(2021YFD2200501)。
关键词
毛竹
表型性状
核心种质
取样策略
评价参数
Phyllostachys edulis
phenotypic traits
core germplasm
sampling strategies
evaluation parameters
作者简介
通信作者:刘广路,E-mail:liuguanglu@icbr.ac.cn。
