摘要
以鱼糜漂洗水回收蛋白质为原料制备具有抗氧化和降血糖活性的蛋白酶解物,对底物质量分数、pH值、酶解时间、温度、加酶量五个因素进行单因素实验和正交优化分析,确定复合蛋白酶水解制备鱼糜漂洗水回收蛋白肽的工艺条件,同时检测蛋白肽的氨基酸组成与分子量分布。结果表明,底物质量分数为3%时,最优酶解方案为:酶解温度45℃、pH值8.0、酶解时间4 h、加酶量4000 U/g prot,该条件下酶解产物水解度达23.62%,多肽含量达23.21 mg/mL。利用该参数制备的蛋白肽氨基酸总量为58.40 g/100 g,疏水性氨基酸含量较高(22.84 g/100 g),占比达39.11%,分子量主要集中于1 kDa以下(占比88.02%)。体外抗氧化和降血糖活性结果表明,蛋白肽对OH·和DPPH·的清除效果较好,半抑制质量浓度(Semi-Inhibitory Concentration,IC_(50))分别为8.28、2.54 mg/mL;对α-葡萄糖苷酶和α-淀粉酶的IC_(50)分别为22.82和26.31 mg/mL。综上,酶解鱼糜漂洗水中回收蛋白质制备的蛋白肽具有较好的体外抗氧化及降血糖效果,研究结果为回收蛋白质的高值化利用提供了新思路。
Proteins recovered from surimi rinsing wastewater were used as raw materials to prepare protein hydrolysates with antioxidant and hypoglycemic activities.Single-factor experiments and orthogonal optimization analysis were conducted with five factors,namely substrate mass fraction,pH,enzymatic hydrolysis time,temperature,and enzyme dosage,to determine the optimal process conditions for the preparation of peptides recovered from surimi rinsing wastewater by hydrolysis with a compound protease.The amino acid composition and molecular weight distribution of the peptides were also analyzed.Results indicated that at a substrate mass fraction of 3%,the optimal enzymatic hydrolysis conditions were as follows:temperature:45℃,pH:8.0,time:4 h,enzyme dosage:4000 U/g prot.Under these conditions,the degree of hydrolysis and peptide content of the obtained enzymatic hydrolysis product were 23.62%and 23.21 mg/mL,respectively.The prepared peptides had a total amino acid content of 58.40 g/100 g and a high hydrophobic amino acid content(22.84 g/100 g)that accounted for 39.11%of the total amino acid content.Molecular weights were mainly concentrated in the<1 kDa range(88.02%of all molecules).Results of in vitro antioxidant and hypoglycemic activity testing revealed that the peptides exerted good scavenging effects on OH·and DPPH·with semi-inhibitory concentrations(IC_(50))of 8.28 mg/mL and 2.54 mg/mL,respectively.The IC_(50)values forα-glucosidase andα-amylase were 22.82 mg/mL and 26.31 mg/mL,respectively.In conclusion,peptides prepared by enzymatic hydrolysis of proteins recovered from surimi rinsing wastewater demonstrated good in vitro antioxidant and hypoglycemic effects.The results of this study can potentially provide a novel approach to the high-value utilization of recovered proteins.
作者
牟黠
冯庆祥
胡杨
尤娟
尹涛
刘茹
MOU Xia;FENG Qingxiang;HU Yang;YOU Juan;YIN Tao;LIU Ru(College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;National R&D Branch Center for Conventional Freshwater Fish Processing(Wuhan),Wuhan 430070,China)
出处
《现代食品科技》
北大核心
2025年第8期61-71,共11页
Modern Food Science and Technology
基金
国家重点研发计划项目(2022YFD2100903)。
关键词
酶解
蛋白肽
氨基酸组成
抗氧化活性
降血糖活性
enzymatic hydrolysis
peptides
amino acid composition
antioxidant activity
hypoglycemic activity
作者简介
牟黠(1998-),女,硕士研究生,研究方向:淡水产品加工及贮藏技术,E-mail:1070590871@qq.com;通讯作者:刘茹(1982-),女,博士,教授,研究方向:水产品加工与贮藏,E-mail:liuru@mail.hzau.edu.cn。
