摘要
【目的】番茄潜叶蛾Tuta absoluta是危害番茄的重大入侵害虫,为了丰富绿色防控技术方法,本研究对番茄潜叶蛾在不同番茄品种上的产卵选择性和内在机理进行了分析。【方法】首先通过田间自然感虫法评价了番茄潜叶蛾在4个番茄品种上的产卵选择性,并通过气质联用(GC-MS)测定了不同品种间的次生代谢产物差异;其次通过饲喂“补充营养”的方式观察了番茄潜叶蛾对脱氢抗坏血酸的行为响应;最后再次利用田间自然感虫法评价了番茄植株外施脱氢抗坏血酸对番茄潜叶蛾产卵选择性的影响。【结果】番茄潜叶蛾在筛选的4个番茄品种上的产卵选择性具有明显差异(P<0.05),其中在台湾黄圣女上的产卵选择性显著(P<0.05)强于甜蜜80,48 h着卵量分别为24.84和7.22粒。台湾黄圣女和甜蜜80的次生代谢产物相对含量存在显著差异(P<0.05),主要差异性物质为脱氢抗坏血酸,且甜蜜80和金红低架王脱氢抗坏血酸的相对含量显著(P<0.05)高于台湾黄圣女和小仙女。将脱氢抗坏血酸添加至10%蜂蜜水(补充营养)中饲喂番茄潜叶蛾成虫发现,番茄潜叶蛾对脱氢抗坏血酸具有负选择性,而且随着脱氢抗坏血酸浓度升高,负选择性响应越强烈,当浓度增加至0.004 mg/mL时,取食补充营养的番茄潜叶蛾数量减少92.00%。对番茄植株喷施脱氢抗坏血酸后,番茄潜叶蛾在4个番茄品种上的产卵量整体呈下降趋势,且脱氢抗坏血酸喷施浓度与番茄品种存在主体间效应(P<0.05)和交互响应(P<0.05),但脱氢抗坏血酸浓度(F=19.92)引起番茄潜叶蛾产卵量差异的效应强于番茄品种(F=4.29),随着喷施的脱氢抗坏血酸浓度升高,番茄潜叶蛾在台湾黄圣女上的着卵量下降速率最快,拟合趋势线的斜率K为﹣20.93,而在甜蜜80上的下降速率最慢,拟合趋势线的斜率K为﹣8.67。【结论】脱氢抗坏血酸可能是形成番茄潜叶蛾对不同番茄品种产卵选择性的关键响应物质之一,且番茄潜叶蛾对该物质的行为响应表现为负选择性。
[Aim]To improve environmentally-friendly prevention and control methods for the tomato leaf miner Tuta absoluta,a major invasive pest of tomato crops.[Methods]The oviposition preferences of the tomato leaf miner for 4 tomato cultivars were evaluated using the natural field infection method,and differences in secondary metabolites among the different cultivars were determined with GC-MS.The behavioral responses of adult tomato leaf miners to hehydroascorbate were examined using the"supplemental nutrition"method.Finally,the field natural infection method was used to evaluate the effect of the external application of hehydroascorbate on oviposition preferences.[Results]There were significant differences in the preference of the tomato leaf miner for each of the 4 tomato cultivars.Preference for the TWHSN variety was significantly stronger than for TM80,with 24.84 and 7.22 eggs laid on each cultivar,respectively,after 48 h.There were significant differences in the secondary metabolites of the TWHSN and TM80 varieties;the main difference was hehydroascorbate,the amount of which was significantly higher in TM80 and JHDJW than in TWHSN and XXN.Adding hehydroascorbate to the supplemental food fed to adult tomato leafminers reduced its palatability a dose-dependent manner.Adding 0.004 mg/mL of hehydroascorbate to supplemental food reduced the number of tomato leafminers feeding on supplemental food by 92.00%.Spraying hehydroascorbate on tomato plants generally reduced the number of eggs laid on all 4 varieties,but there was a between-subject effect(P<0.05)and an interaction(P<0.05)between hehydroascorbate concentration and tomato cultivar.However,hehydroascorbate concentration was a more significant factor(F=19.92)than tomato cultivar(F=4.29).The most rapid decline in the number of eggs laid as the hehydroascorbate concentration increased was on TWHSN,with a slope K of the fitted trend line of﹣20.93.The slowest decline in the number of eggs laid was on TM80,with a slope K of the fitted trend line of﹣8.67.[Conclusion]Hehydroascorbate could be a key substance reducing the attractiveness of tomato plants to the tomato leaf miner for oviposition.
作者
魏玉红
袁伟宁
张美娇
郭致杰
WEI Yu-Hong;YUAN Wei-Ning;ZHANG Mei-Jiao;GUO Zhi-Jie(Institute of Plant Protection,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China;Gansu Agricultural Pest Natural Enemy Engineering Research Center,Lanzhou 730070,China)
出处
《应用昆虫学报》
北大核心
2025年第4期1014-1024,共11页
Chinese Journal of Applied Entomology
基金
国家重点研发项目(2021YFD1400200)
甘肃省农业科学院重点研发项目(2022GAAS44)。
关键词
番茄潜叶蛾
番茄品种
产卵选择性
化感物质
寄主选择偏好性
Tuta absoluta
tomato variety
ovipositional tropism
chemosensory substances
host selection preference
作者简介
第一作者:魏玉红,E-mail:727339710@qq.com;通讯作者:郭致杰,E-mail:guozhijie@gsagr.ac.cn。
