摘要
目的比较猕猴海南亚种(Macaca mulatta brevicaudus)的体质量和血液生理生化指标,为后续实验动物培育提供参考。方法本研究以陵水南湾猕猴自然保护区内的180只野生猕猴海南亚种(雌性和雄性)为对象,使用电子秤测量其体质量,同时采集猕猴血液样本,采用全自动血细胞分析仪检测血常规指标(RBC、HGB、WBC、PLT等),并用全自动生化分析仪测定生化指标(ALT、AST、GLU、BUN等)。数据分析通过SPSS 18.0软件进行描述性统计,并运用t检验比较不同性别和年龄段猕猴的体质量及血液指标差异。结果研究结果显示,在成年野生猕猴海南亚种中,雄性体质量显著高于雌性(P<0.05)。然而,雌性和雄性猕猴的全血细胞计数结果未见显著差异(P>0.05)。在血液生化指标方面,雌性和雄性猕猴海南亚种之间乳酸脱氢酶(LDH)和总胆红素(TBIL)水平差异显著(P<0.05),而其他生化指标则无显著差异。结论本研究获得了野生猕猴海南亚种体质量和血液生理生化指标基础数据,为后续猕猴海南亚种实验动物化培育提供参考。
Objective To determine the body weights and blood physiological and biochemical indicators in the Macaca mulatta brevicaudus(M.m.brevicaudus),to provide a reference for the breeding of experimental animals.MethodsA total of 180 wild M.m.brevicaudus(female and male)from the South Bay Macaque Reserve in Lingshui were selected as the research subjects.Body weights were measured using electronic scales and blood samples were collected.Routine blood indicators(red blood cell count,hemoglobin,white blood cell count,and platelet count)were detected using an automated blood cell analyzer,and biochemical indicators(alanine aminotransferase,aspartate aminotransferase,glucose,blood urea nitrogen)were measured using an automated biochemical analyzer.Data were analyzed using SPSS 18.0 software for descriptive statistics,and differences in body weights and blood indicators between sexes and age groups were compared using t-tests.ResultsAdult male wild M.m.brevicaudus were significantly heavier than females(P<0.05);however,there were no significant differences in complete blood cell counts between female and male macaques(P>0.05).In terms of blood biochemical indicators,lactate dehydrogenase and total bilirubin levels differed significantly between female and male M.m.brevicaudus(P<0.05),but there were no significant differences in any other biochemical indicators.Conclusions This study established baseline data on the body weights and blood physiological and biochemical indicators of wild M.m.brevicaudus,providing a reference for their future breeding as experimental animals.
作者
刘保臻
王军
孙瑞萍
伍乘风
赵鑫源
赵建国
袁靖丽
LIU Baozhen;WANG Jun;SUN Ruiping;WU Chengfeng;ZHAO Xinyuan;ZHAO Jianguo;YUAN Jingli(Innovation Center of Academician Xia Xianzhu’s Team,Key Laboratory of Tropical Animal Breeding and Disease Research,Institute of Animal Science and Veterinary Medicine,Hainan Academy of Agricultural Sciences,Haikou 571100,China;College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642;School of Life Sciences,Sun Yat-sen University,Guangzhou 510275;Sanya Institute,Hainan Academy of Agricultural Sciences,Sanya 572019;State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Stem Cell and Regenerative Medicine Innovation Institute,Chinese Academy of Sciences,Beijing 100101)
出处
《中国比较医学杂志》
北大核心
2025年第8期67-77,共11页
Chinese Journal of Comparative Medicine
基金
海南省科研院所技术创新专项(SQKY2022-0016)
海南省农业科学院引进人才科研启动基金项目(HNXM2024 RCQD06)。
关键词
猕猴海南亚种
野生动物
体质量
血液生化
乳酸脱氢酶
Macaca mulatta brevicaudus
wild animals
body weight
blood biochemistry
lactate dehydrogenase
作者简介
刘保臻(1994-),女,在读博士研究生,研究方向:食蟹猴生殖生理。E-mail:bushiaikugui@126.com;通信作者:袁靖丽(1991-),女,博士,助理研究员,研究方向:实验动物与疾病研究。E-mail:13250732023@163.com。
