摘要
细胞内pH值在生理和病理过程中起着非常重要的作用,因此建立精确、灵敏检测细胞pH值的方法显得十分重要。本文以生物质芹菜叶为原料,通过溶剂热法合成表面氨基化的近红外荧光碳点(CDs),然后将其与异硫氰酸荧光素(FITC)通过共价偶联构建一种单激发双发射的近红外比率荧光探针(CDs-F)。结果显示,pH值在5.0~9.0时,CDs-F发射峰有响应且与其呈良好线性关系,同时有较低的细胞毒性。该比率荧光纳米探针可用于pH值的检测及细胞内微小pH值的比率荧光成像。
Intracellular pH plays a very important role in physiological and pathological processes,so it is important to establish an accurate and sensitive method to detect cellular pH.In this paper,biomass celery leaves are used as raw materials to synthesize amino-infrared fluorescent carbon dots(CDs)with surface amination by solvothermal method,and then covalently coupled with fluorescein isothiocyanate(FITC)to construct a single-excitation and double-emission near-infrared ratiometric fluorescent probe(CDs-F).The results show that when the pH value is 5.0 to 9.0,the CDs-F emission peak has a good linear relationship with the CDs-F emission peak,and has low cytotoxicity.The ratiometric fluorescence nanoprobe can be used for the detection of pH value and the ratio fluorescence imaging of intracellular micropH value.
作者
杨克琴
谢婷婷
YANG Keqin;XIE Tingting(Guilin Normal University,Guilin 541199,China)
出处
《生物化工》
2025年第4期18-23,29,共7页
Biological Chemical Engineering
基金
广西自然科学基金青年项目(2023GXNSFBA026314)
广西高校中青年教师(科研)基础能力提升项目(2021KY0939)。
关键词
碳点
比率荧光探针
细胞成像
PH值
carbon dots
ratio fluorescence probe
cell imaging
pH value
作者简介
通信作者:杨克琴(1983-),女,河南信阳人,博士,副教授,研究方向为纳米材料与生物传感。E-mail:154333176@qq.com。
