摘要
文章采用热诱导法制备了大豆分离蛋白(Soybean protein isolate,SPI)-壳聚糖(Chitosan,CS)纳米复合颗粒,并以此为乳化剂制备出负载叶黄素酯的Pickering乳液,分析SPI与CS的复合比例对复合颗粒粒径、Zeta电位和水接触角的影响。通过单因素实验和响应面分析,优化了叶黄素酯Pickering乳液的包埋率。结果表明,随着CS比例的增加,复合颗粒的粒径、Zeta电位逐渐增大,水接触角逐渐减小,复合颗粒的乳化能力发生变化。SPI-CS复合颗粒比例为5:1、水油比为2:3时,制备出的叶黄素酯Pickering乳液在4℃放置28 d后乳化指数为0,乳液稳定性好。SPI-CS复合颗粒在质量分数0.7%、剪切速率8000 r/min、乳化温度50℃条件下制备的叶黄素酯Pickering乳液对叶黄素酯的包埋率可达91.52%。SPI-CS复合颗粒制备的叶黄素酯Pickering乳液拓展了叶黄素酯高值化应用。
In this study,soybean protein isolate(SPI)-chitosan(CS)nanocomposite particles were prepared using a thermal induction method and employed as emulsifiers to prepare Pickering emulsion loaded with lutein ester.The effects of the composite ratio of SPI to CS on particle size,Zeta potential,and water contact angle of the composite particles were analyzed.Through single factor experiment and response surface analysis,the embedding efficiency of lutein ester Pickering emulsion was optimized.The experimental results indicate that as the CS ratio increases,both the particle size and Zeta potential of the composite particles gradually increase,while the water contact angle gradually decreases.Consequently,the emulsifying ability of the composite particles was affected.When the ratio of SPI-CS composite particles was 5∶1 and the water-oil ratio was 2∶3,the emulsion index of the prepared lutein ester Pickering emulsion was 0 after 28 days at 4℃,and the emulsion stability was ideal.The embedding efficiency of lutein ester Pickering emulsion prepared under the conditions of SPI-CS composite particles concentration of 0.7%,shear rate of 8000 r/min and emulsification temperature of 50℃ was 91.52%.The Pickering emulsion of lutein ester stabilized by SPI-CS composite particles has expanded the high-value applications of lutein ester.
作者
王利宽
武耐英
高伟
徐建中
安晓东
宋伟光
WANG Likuan;WU Naiying;GAO Wei;XU Jianzhong;AN Xiaodong;SONG Weiguang(School of Materials Science and Engineering,Hebei University of Engineering,Handan 056038,China;Chenguang Biotechnology Co.,Ltd.,Handan 057250,China;Key Laboratory of Comprehensive Utilization of Plant Resources,Handan 057250,China)
出处
《食品科技》
北大核心
2025年第7期225-233,共9页
Food Science and Technology
基金
邯郸市科学技术研究与发展计划项目(21422012320,22313014014)。
作者简介
王利宽(1998-),男,硕士研究生,研究方向为天然产物应用;通信作者:武耐英;通信作者:高伟。
