摘要
[目的]探究玉米种质资源对瘤黑粉病的真实抗性。[方法]利用人工接种方法连续多年对新选育的玉米自交系进行抗瘤黑粉病精准鉴定和评价,并通过F2分离群体抗感单株分离比例和适合性检验,初步分析高抗材料的抗性遗传规律。[结果]在32份待鉴定材料中,20份玉米自交系对瘤黑粉病具有抗性,占鉴定材料总数的62.5%,其中高抗瘤黑粉病自交系6份,中抗瘤黑粉病自交系9份,抗瘤黑粉病自交系5份;12份玉米自交系对瘤黑粉病表现为感病,占鉴定材料总数的37.5%。根据F2分离群体抗感植株分离比例和适合性测验,分析6份高抗玉米自交系抗瘤黑粉病遗传规律,结果表明,可能是1对显性单基因控制着新选育自交系Z17HEB-82、Z17HEB-93、Z17HEB-100和Z17HEB-107对玉蜀黍黑粉菌的抗性。[结论]该研究结果可为今后我国玉米瘤黑粉病抗性种质的引进及利用提供重要参考。
[Objective]To explore the true resistance of maize germplasm resources to common smut.[Method]New maize inbred lines were precisely identified and evaluated for resistance to common smut by using artificial inoculation methods for consecutive years.The genetic law of resistance of highly resistant materials was preliminarily analyzed through the segregation ratio analysis of the F 2 population and adaptability tests of individual resistant and sensitive plants.[Result]A total of 20 resistance materials of 32 maize inbred lines were screened,accounting for 62.5%of the total identified materials,among which,6 materials were highly resistant,9 materials were medium resistant and 5 materials were resistant to common smut.A total of 12 sensitive materials were screened,accounting for 37.5%of the total identified materials.Genetic analysis of resistance of 6 highly resistance materials to maize common smut was carried out according to the separation ratio and suitability of resistant and susceptible plants by using F 2 population.The results showed that resistance of Z17HEB-82、Z17HEB-93、Z17HEB-100 and Z17HEB-107 inbred lines to Ustilago zeae may be controlled by one pair of dominant single gene.[Conclusion]The results can provide important references for the introduction and utilization of maize common smut resistant germplasm in the future in China.
作者
鞠洪峰
成善彬
李华青
熊万光
汪广亮
宋明川
肖明纲
JU Hong-feng;CHENG Shan-bin;LI Hua-qing(Biocentury Transgene(China)Co.,Ltd.,Shenzhen,Guangdong 518017)
出处
《安徽农业科学》
2025年第17期10-13,共4页
Journal of Anhui Agricultural Sciences
基金
黑龙江省博士后基金项目(LB-Z14186)。
关键词
玉米自交系
瘤黑粉病
精准鉴定
抗性评价
遗传分析
Maize inbred line
Common smut
Precision identification
Resistance evaluation
Genetic analysis
作者简介
鞠洪峰(1980-),男,吉林镇赉人,初级农艺师,从事玉米抗病种质资源鉴定及抗病新品种培育工作;通信作者:肖明纲,副研究员,博士,从事玉米抗病种质资源鉴定及抗性遗传分析工作。
